Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints

	Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints
	Ding, Lingling 1,3; Zhou, Qixing 1,2; Wang, Lin 1,3; Zhang, Qianru 1
刊名	AFRICAN JOURNAL OF BIOTECHNOLOGY
	2011-01-24
卷号	10 期号:4 页码:589-600
关键词	Community Structure Pcr-dgge 16s Ribosomal Dna Wastewater Treatment Plant (Wwtp) Activated Sludge
ISSN号	1684-5315
英文摘要	The microbial community composition and dominant bacterial populations in anoxic-oxic activated sludge from a full-scale wastewater treatment plant (WWTP), were investigated with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) coupled with sequence analysis of 16S rRNA gene fragments from dominant bands. The bacterial ecological distribution at different parts of each reactor, the dynamics of the bacterial community structure over the total process course and temporal dynamics were obtained by comparing the DGGE patterns. Differences were noted between three sections of the reactors and also two reactors within the entire process of the system. Temporal variations in bacterial composition of the activated sludge in each reactor were responded to their circumstances. The prominent DGGE bands were excised and sequenced in order to identify the predominantly present and active bacterial populations. Here, combined with phylogenetic analysis of the DGGE fingerprints, the four major bacterial lineages can be revealed: alpha, beta, y- Proteobacteria, and the phylum Firmicutes (low G+C Gram-positive). The 16S rDNA sequence analysis indicated that the DGGE bands of dominant bacterial from this plant harbored sequences of possible nitrogen remover with potential aerobic denitrification / heterotrophic nitrification.
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ACADEMIC JOURNALS
WOS记录号	WOS:000286526500018
内容类型	期刊论文
源URL	[http://210.72.129.5/handle/321005/121709]
专题	中国科学院沈阳应用生态研究所
通讯作者	Zhou, Qixing
作者单位	1.Chinese Acad Sci, Inst Appl Ecol, Key Lab Terr Ecol Proc, Shenyang 110016, Peoples R China 2.Nankai Univ, Coll Environm Sci & Engn, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ding, Lingling,Zhou, Qixing,Wang, Lin,et al. Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(4):589-600.
APA	Ding, Lingling,Zhou, Qixing,Wang, Lin,&Zhang, Qianru.(2011).Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(4),589-600.
MLA	Ding, Lingling,et al."Dynamics of bacterial community structure in a full-scale wastewater treatment plant with anoxic-oxic configuration using 16S rDNA PCR-DGGE fingerprints".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.4(2011):589-600.