High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)

doi:10.1038/s41598-018-32015-y

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)
	Wang, Xiuliang 1,2,4; Chen, Zhihang 1,2,3; Li, Qiuying1,2,3 ; Zhang, Jie1,2,4 ; Liu, Shun 1,2,3; Duan, Delin 1,2,4
刊名	SCIENTIFIC REPORTS
	2018-09-11
卷号	8 页码:10
ISSN号	2045-2322
DOI	10.1038/s41598-018-32015-y
通讯作者	Duan, Delin(dlduan@qdio.ac.cn)
英文摘要	Saccharina japonica is one of the most important marine crops in China, Japan and Korea. Candidate genes associated with blade length and blade width have not yet been reported. Here, based on SLAF-seq, the 7627 resulting SNP loci were selected for genetic linkage mapping to 31 linkage groups with an average spacing of 0.69 cM, and QTL analyses were performed to map the blade length and blade width phenotypes of S. japonica. In total, 12 QTLs contributing to blade length and 10 to width were detected. Some QTL intervals were detected for both blade length and width. Additive alleles for increasing blade length and width in S. japonica came from both parents. After the QTL interval regions were comparatively mapped to the current reference genome of S. japonica (MEHQ00000000), 14 Tic20 (translocon on the inner envelope membrane of chloroplast) genes and three peptidase genes were identified. RT-qPCR analysis showed that the transcription levels of four Tic20 genes were different not only in the two parent sporophytes but also at different cultivation times within one parent. The SNP markers closely associated with blade length and width could be used to improve the selection efficiency of S. japonica breeding.
资助项目	NSFC[31772848] ; NSFC[31272660] ; Key Lab of Marine Bioactive Substance and Modern Analytical Technique, SOA ; National Key Technology Research and Development Program[2013BAB01B01] ; Ocean Public Welfare Scientific Research Project[201405040] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Shandong Key Sci-Technology Research Project[2016ZDJS06B2]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000444278400025
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/160259]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Duan, Delin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100093, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiuliang,Chen, Zhihang,Li, Qiuying,et al. High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)[J]. SCIENTIFIC REPORTS,2018,8:10.
APA	Wang, Xiuliang,Chen, Zhihang,Li, Qiuying,Zhang, Jie,Liu, Shun,&Duan, Delin.(2018).High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta).SCIENTIFIC REPORTS,8,10.
MLA	Wang, Xiuliang,et al."High-density SNP-based QTL mapping and candidate gene screening for yield-related blade length and width in Saccharina japonica (Laminariales, Phaeophyta)".SCIENTIFIC REPORTS 8(2018):10.