A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection

doi:10.1016/j.dci.2017.12.018

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection
	Yang, Feifei 1; Li, Shihao 1; Li, Fuhua 1,2; Xiang, Jianhai1,2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2018-04-01
卷号	81 页码:303-311
关键词	Cuticle protein WSSV infection VP24 Litopenaeus vannamei
ISSN号	0145-305X
DOI	10.1016/j.dci.2017.12.018
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
英文摘要	White spot syndrome virus (WSSV) is a major viral pathogen in global shrimp farming, causing huge economic damage. Through penetrating the outer surface of the target tissues, WSSV enters into the cells of the target tissue to complete the replication process in the host. In the present study, a cuticle protein gene from Litopenaeus vannamei, designated as LvAMP13.4, was identified and proved to be involved in WSSV invasion. The deduced amino acid sequence of LvAMP13.4 contained a signal peptide and a conserved chitin-binding domain type 4 (ChBD4). This cuticle protein gene was mainly expressed in stomach, gill and epidermis. The expression level of LvAMP13.4 was significantly changed during WSSV infection. Silencing of LvAMP13.4 by dsRNA interference apparently reduced the mortality rate and the WSSV copy number in shrimp upon WSSV infection. Furthermore, yeast two-hybrid system and Co-IP assay were performed to confirm that LvAMP13.4 could interact with the major envelop protein VP24 of WSSV. These data indicated that LvAMP13.4 was involved in the invasion process of WSSV through interaction with VP24. The present results could provide new insights for us in understanding the role of host cuticle proteins during virus invasion. (C) 2017 Elsevier Ltd. All rights reserved.
资助项目	NSFC-ISF Research Program of National Natural Science Foundation of China[31461143007] ; China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361]
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000424726900031
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158063]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Yang, Feifei,Li, Shihao,Li, Fuhua,et al. A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,81:303-311.
APA	Yang, Feifei,Li, Shihao,Li, Fuhua,&Xiang, Jianhai.(2018).A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,81,303-311.
MLA	Yang, Feifei,et al."A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 81(2018):303-311.