CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1

doi:10.2174/1574892813666180628170240

CORC > 海洋研究所 > 中国科学院海洋研究所

	CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1
	Su, Shuonan 1; Xu, Huanli 1; Chen, Xiaoliang 2; Qiao, Gan 1; Farooqi, Ammad A.3; Tian, Ye 1; Yuan, Ru 1; Liu, Xiaohui 1; Li, Cong 1; Li, Xiao 1
刊名	RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY
	2018
卷号	13 期号:3 页码:360-367
关键词	Enolase1 migration and invasion protein-protein interaction recombinant CS5931
ISSN号	1574-8928
DOI	10.2174/1574892813666180628170240
通讯作者	Lin, Xiukun(xiukunlin@126.com)
英文摘要	Background: CS5931, a novel marine peptide, was extracted and purified from the sea squirt Ciona savignyi. Our previous studies showed that recombinant CS5931 can significantly inhibit tumor growth both in vitro and in vivo. However, its molecular targets have not been elucidated. Methods: The target of the recombinant CS5931 was identified by pull-down/SDS-PAGE/MS approaches and confirmed by Western blot and surface plasmon resonance analysis. Transwell experiments were used to detect whether the recombinant CS5931 inhibited cancer migration and invasion via enolase 1. Dot blotting analysis was used to detect the effect of CS5931 on the interaction of enolase 1 and plasminogen, as well as enolase 1 and uPA/uPAR. Results: Enolase 1 was identified as the molecular target interacting with the recombinant CS5931. Transwell experiment showed that the recombinant CS5931 was able to inhibit migration and invasion of HCT116 cells and enolase 1 overexpression reversed the effects of the recombinant CS5931 on migration and invasion of cancer cells. Dot blotting analysis revealed that the recombinant CS5931 interfered with the interaction among enolase 1 and plasminogen as well as enolase 1 and uPA/uPAR. Conclusion: Our present study showed that the recombinant CS5931 could inhibit tumor invasion and matastasis via interacting with enolase 1, suggesting that the new marine polypeptide CS5931 possesses the potential to be developed as a novel anticancer agent.
资助项目	Natural Science Foundation of China[81773776] ; Natural Science Foundation of China[81573457] ; Natural Science Foundation of China[81774191] ; Beijing Natural Science Foundation[7172031] ; Beijing Natural Science Foundation[7172029] ; Shandong Provincial Natural Science Foundation[2016GGD03015] ; Zibo Development Program of Sci. & Tech, in Shandong, China[2016KJ100048] ; National Innovative Drug Development Project of China[2014ZX-09102043-001]
WOS研究方向	Oncology ; Pharmacology & Pharmacy
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000441337300007
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/159686]
专题	中国科学院海洋研究所
通讯作者	Lin, Xiukun
作者单位	1.Capital Med Univ, Dept Pharmacol, Beijing 100069, Peoples R China 2.Shanxi Datong Univ, Sch Med, Datong 037009, Peoples R China 3.Inst Biomed & Genet Engn, Islamabad, Pakistan 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Su, Shuonan,Xu, Huanli,Chen, Xiaoliang,et al. CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1[J]. RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY,2018,13(3):360-367.
APA	Su, Shuonan.,Xu, Huanli.,Chen, Xiaoliang.,Qiao, Gan.,Farooqi, Ammad A..,...&Lin, Xiukun.(2018).CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1.RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY,13(3),360-367.
MLA	Su, Shuonan,et al."CS5931, A Novel Marine Polypeptide, Inhibits Migration and Invasion of Cancer Cells Via Interacting with Enolase 1".RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY 13.3(2018):360-367.