Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China

doi:10.1007/s13213-018-1328-0

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	Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China
	Fu, Songzhe 1; Wang, Liping 2; Tian, Huiqin 3; Wei, Dawei 4; Liu, Ying1
刊名	ANNALS OF MICROBIOLOGY
	2018-04-01
卷号	68 期号:4 页码:175-184
关键词	Vibrio parahaemolyticus AHPND PacBio pirAB(vp) Prophage Type VI secretion system
ISSN号	1590-4261
DOI	10.1007/s13213-018-1328-0
通讯作者	Fu, Songzhe(fusongzhe@hotmail.com)
英文摘要	Acute hepatopancreatic necrosis disease (AHPND) outbreaks in cultured shrimps were identified in Zhangpu, China. One Vibrio parahaemolyticus strain PB1937 was isolated from the cultured shrimps and was confirmed as a causative agent of the AHPND outbreak by employing Koch's four postulates. Challenge tests with 10(6) cells ml(-1) of strain PB1937 caused 100% mortality of shrimps, indicating it had sufficient virulence to cause the outbreak. Phylogenomic analysis revealed a clear divergence between PB1937 and 14 publicly available V. parahaemolyticus strains and divided 11 AHPND-causing strains into six genomic clusters. Prophage profiling of above strains revealed strong correlations with their genomic relationship, while Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) were almost absent in the genomes. The binary toxin gene pirAB(vp) directly related to the development of AHPND was found in a 70-kb plasmid p1937-1 in PB1937 but was absent in a 78-kb novel plasmid p1937-2, which shared 46% sequence similarity with p1937-1. Comparative genomic analysis revealed that PB1937 has a novel truncated type VI secretion system (T6SS1) which possibly affects its antibacterial activity. In addition, three novel genomic islands were reported. The analysis of the genomes gave some clues regarding the correlation of virulence with its genomic trait for the AHPND strains.
资助项目	National Key R&D Program of China[2017YFD0701700] ; National Natural Science Foundation of China[31672673] ; National Natural Science Foundation of China[31472312]
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000429125500002
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158773]
专题	中国科学院海洋研究所
通讯作者	Fu, Songzhe
作者单位	1.Dalian Ocean Univ, Coll Marine Technol & Environm, 52 Heishijiao St, Dalian 116023, Peoples R China 2.State Oceanog Adm China, Inst Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Northwest Agr & Forestry Univ, Coll Life Sci, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Fu, Songzhe,Wang, Liping,Tian, Huiqin,et al. Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China[J]. ANNALS OF MICROBIOLOGY,2018,68(4):175-184.
APA	Fu, Songzhe,Wang, Liping,Tian, Huiqin,Wei, Dawei,&Liu, Ying.(2018).Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China.ANNALS OF MICROBIOLOGY,68(4),175-184.
MLA	Fu, Songzhe,et al."Pathogenicity and genomic characterization of Vibrio parahaemolyticus strain PB1937 causing shrimp acute hepatopancreatic necrosis disease in China".ANNALS OF MICROBIOLOGY 68.4(2018):175-184.