Directional mechanical stability of Bacteriophage φ29 motor’s 3WJ-pRNA: Extraordinary robustness along portal axis

doi:10.1126/sciadv.1601684

	Directional mechanical stability of Bacteriophage φ29 motor’s 3WJ-pRNA: Extraordinary robustness along portal axis
	Xu ZH(徐钟河); Li JY(李敬源); Xu, Z., Y. Sun, J. K. Weber, Y. Cao, W. Wang, D. Jasinski, P. Guo, R. Zhou and J. Li
刊名	SCIENCE ADVANCES
	2017
卷号	3 期号:5 页码:e1601684
ISSN号	2375-2548
DOI	10.1126/sciadv.1601684
文献子类	Article
英文摘要	The molecular motor exploited by bacteriophage phi 29 to pack DNA into its capsid is regarded as one of the most powerful mechanical devices present in viral, bacterial, and eukaryotic systems alike. Acting as a linker element, a prohead RNA (pRNA) effectively joins the connector and ATPase (adenosine triphosphatase) components of the phi 29 motor. During DNA packing, this pRNA needs to withstand enormous strain along the capsid's portal axis-how this remarkable stability is achieved remains to be elucidated. We investigate the mechanical properties of the phi 29 motor's junction (3WJ)-pRNA using a combined steered molecular dynamics and atomic force approach. The 3WJ exhibits strong resistance to stretching along its coaxial helices, demonstrating its super structural robustness. This resistance disappears, however, when external forces are applied to the transverse directions. From a molecular standpoint, we demonstrate that this direction-dependent stability can be attributed to clamps that cooperate and generate mechanical resistance in the pRNA's coaxial direction. Our results suggest that the asymmetric nature of the 3WJ's mechanical stability is entwined with its biological function: Enhanced rigidity along the portal axis is likely essential to withstand the strain caused by DNA condensation, and flexibility in other directions should aid in the assembly of the pRNA and its association with other motor components.
WOS关键词	MOLECULAR-DYNAMICS SIMULATIONS ; DNA PACKAGING MOTOR ; PARTICLE MESH EWALD ; FORCE SPECTROSCOPY ; BIOMOLECULAR SIMULATIONS ; FUNCTIONAL DOMAINS ; INTERNAL FORCE ; 3-WAY JUNCTION ; VIRAL-RNA ; IN-VITRO
WOS研究方向	Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000419752300003
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/284928]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Xu ZH,Li JY,Xu, Z., Y. Sun, J. K. Weber, Y. Cao, W. Wang, D. Jasinski, P. Guo, R. Zhou and J. Li. Directional mechanical stability of Bacteriophage φ29 motor’s 3WJ-pRNA: Extraordinary robustness along portal axis[J]. SCIENCE ADVANCES,2017,3(5):e1601684.
APA	徐钟河,李敬源,&Xu, Z., Y. Sun, J. K. Weber, Y. Cao, W. Wang, D. Jasinski, P. Guo, R. Zhou and J. Li.(2017).Directional mechanical stability of Bacteriophage φ29 motor’s 3WJ-pRNA: Extraordinary robustness along portal axis.SCIENCE ADVANCES,3(5),e1601684.
MLA	徐钟河,et al."Directional mechanical stability of Bacteriophage φ29 motor’s 3WJ-pRNA: Extraordinary robustness along portal axis".SCIENCE ADVANCES 3.5(2017):e1601684.