An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase

doi:10.1002/asia.201801601

	An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase
	Tang, Bo 2; Li, Lu 2; Shangguan, Huijuan 3; Wan, Chuyun 3; Chai, Zhaofei 1; Li, Ya 3; Deng, Qianchun 3; Shi, Jie 2,3
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2019-03-15
卷号	14 期号:6 页码:847-852
关键词	aggregation-induced emission fluorescence fluorescent probes host-guest systems alpha-amylase
ISSN号	1861-4728
DOI	10.1002/asia.201801601
英文摘要	alpha-Amylase, an essential biomarker in pancreas related diseases and perform a major role in carbohydrates metabolism. Hence, monitoring the dynamic changes of alpha-amylase is crucial for better clinical diagnosis of diseases. However, the existing methods are suffered from low sensitivity, time consumption and indirect assay with aid of tool enzyme or inhibitor of competitive substrates, the rapid and non-destructive sensing of alpha-amylase in biological samples was highly desired. In this work, a very simple tetraphenylethylene motif and gamma-cyclodextrin based supramolecular fluometric sensing system was firstly established. This system has no emission signal in aqueous media for the freely rotation of phenyl rings in the cavity of gamma-cyclodextrin, but the AIE residues can be released in to water after the alpha-amylase hydrolysing gamma-cyclodextrin, then turn on the fluorescence. In this system, the detection limit is calculated to be 0.007 U mL(-1) in MES buffer with a linear range of 0-0.35 U mL(-1), having excellent selectivity to alpha-amylase compared to other proteins. At last, our probe can be applied to the quantitative analysis of alpha-amylase in human serum, showing potential in point of care testing.
资助项目	National Science Foundation of China[21535004] ; National Science Foundation of China[91753111] ; National Science Foundation of China[31872896] ; National Science Foundation of China[31501423] ; National Science Foundation of China[31501409] ; National Science Foundation of China[21390411] ; Young Elite Scientists Sponsorship Program by CAST[2017QNRC001] ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences[CAAS-ASTIP-2013-OCRI]
WOS关键词	2-PHOTON FLUORESCENT-PROBE ; LIPASE ; DIAGNOSIS ; SERUM ; PANCREATITIS ; AIEGEN ; SENSE ; ASSAY
WOS研究方向	Chemistry
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000461836500022
资助机构	National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13852]
专题	中国科学院武汉物理与数学研究所
通讯作者	Tang, Bo
作者单位	1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 2.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Minist Educ, Key Lab Mol & Nano Probes, Jinan 250014, Shandong, Peoples R China 3.Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr, Key Lab Oilseeds Proc,Minist Agr, Wuhan 430062, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Tang, Bo,Li, Lu,Shangguan, Huijuan,et al. An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase[J]. CHEMISTRY-AN ASIAN JOURNAL,2019,14(6):847-852.
APA	Tang, Bo.,Li, Lu.,Shangguan, Huijuan.,Wan, Chuyun.,Chai, Zhaofei.,...&Shi, Jie.(2019).An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase.CHEMISTRY-AN ASIAN JOURNAL,14(6),847-852.
MLA	Tang, Bo,et al."An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase".CHEMISTRY-AN ASIAN JOURNAL 14.6(2019):847-852.