Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI

doi:10.1016/j.ijcard.2019.03.025

	Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI
	Zhang, Ye 5; He, Shufang 5; Cheng, Xueying 5; Huang, Jun 5; Xu, Shijin 5; Cai, Aoling 4; Zhong, Kai 2; Qian, Junchao 2; Xiong, Wei 3; Du, Feng 3
刊名	INTERNATIONAL JOURNAL OF CARDIOLOGY
	2019-06-15
卷号	285 页码:59-64
关键词	Functional MRI Myocardial ischemia-reperfusion injury Substantia gelatinosa Spinal cord Patch clamp recording
ISSN号	0167-5273
DOI	10.1016/j.ijcard.2019.03.025
英文摘要	Background: Myocardial ischemia and reperfusion-evoked spinal reflexes involve nociceptive signals that trigger neuronal excitation through cardiac afferents, projecting into the thoracic spinal cord. Ischemic preconditioning (IPC) involves brief episodes of sublethal ischemia and reperfusion enhances the resistance of the myocardium to subsequent ischemic insults. This study investigated the effects of IPC on ischemia-reperfusion (I/R) stimulation induced activation in the thoracic spinal cord of rats. Methods: A new remotely controlled I/R model was established. The infarct size was determined as a percentage of area at risk (IS/AAR) and arrhythmia scores were evaluated. Non-invasive in vivo fMRI was used for signal quantitative analysis of thoracic spinal spatiotemporal. The role of IPC on the excitability of substantia gelatinosa (SG) neurons was assessed by spinal patch clamp recording technique. The altered expressions of c-Fos, SP, and CGRP in T-4 segment were detected by immunohistochemical staining. Results: The novel I/Rmodel was induced successfully and reliably utilized, and IPC treatment markedly reduced the myocardial infarct size. fMRI analysis revealed that IPC reduced the increased BOLD signals induced by prolonged ischemia-reperfusion. Patch clamp recording showed that IPC treatment reversed the enhanced excitability of SG neurons during I/R treatment. The results of immunofluorescent staining indicated that IPC mitigated the I/R-induced dramatic increase of c-Fos, and reduced the release of SP and CGRP in dorsal horns of spinal cord. Conclusions: These results suggested that IPC suppressed neuronal activation induced by I/R stimuli in rat thoracic spinal cord using spinal cord fMRI and patch clamp recording techniques. (C) 2019 Elsevier B.V. All rights reserved.
资助项目	Natural Science Foundation of Higher Education Institutions of Anhui Province, China[KJ2014ZD16] ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China[KJ2017A172] ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China[Y6Y0021004]
WOS关键词	SUBSTANCE-P RELEASE ; GELATINOSA NEURONS ; CARDIAC ISCHEMIA ; AFFERENT ; BRADYKININ ; BOLD ; FMRI ; EXCITABILITY ; MODULATION ; MECHANISMS
WOS研究方向	Cardiovascular System & Cardiology
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000464016000011
资助机构	Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Natural Science Foundation of Higher Education Institutions of Anhui Province, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China ; Youth Innovation Promotion Association of Chinese Academy of Sciences, China
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13725]
专题	中国科学院武汉物理与数学研究所
通讯作者	Zhang, Ye; He, Shufang
作者单位	1.Second Hosp Shijiazhuang, Shijiazhuang 050051, Hebei, Peoples R China 2.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Neurodegenerat Disorder Res Ctr, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst,Wuhan Inst Phy, Wuhan 430071, Hubei, Peoples R China 5.Anhui Med Univ, Dept Anesthesiol, Hosp 2, 678 Furong Rd, Hefei 230601, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ye,He, Shufang,Cheng, Xueying,et al. Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI[J]. INTERNATIONAL JOURNAL OF CARDIOLOGY,2019,285:59-64.
APA	Zhang, Ye.,He, Shufang.,Cheng, Xueying.,Huang, Jun.,Xu, Shijin.,...&Huang, Cheng.(2019).Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI.INTERNATIONAL JOURNAL OF CARDIOLOGY,285,59-64.
MLA	Zhang, Ye,et al."Effect of myocardial ischemic preconditioning on ischemia-reperfusion stimulation-induced activation in rat thoracic spinal cord with functional MRI".INTERNATIONAL JOURNAL OF CARDIOLOGY 285(2019):59-64.