Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes | |
Gao, Fan-Xiang1,2; Wang, Yang1; Zhang, Qi-Ya1; Mou, Cheng-Yan1,2; Li, Zhi1; Deng, Yuan-Sheng1,2; Zhou, Li1; Gui, Jian-Fang1 | |
刊名 | BMC GENOMICS |
2017-07-24 | |
卷号 | 18期号:1页码:19 |
关键词 | Gibel carp Herpesvirus Transcriptome Interferon Disease resistance Immune response |
ISSN号 | 1471-2164 |
DOI | 10.1186/s12864-017-3945-6 |
英文摘要 | Background: Gibel carp is an important aquaculture species in China, and a herpesvirus, called as Carassius auratus herpesvirus (CaHV), has hampered the aquaculture development. Diverse gynogenetic clones of gibel carp have been identified or created, and some of them have been used as aquaculture varieties, but their resistances to herpesvirus and the underlying mechanism remain unknown. Results: To reveal their susceptibility differences, we firstly performed herpesvirus challenge experiments in three gynogenetic clones of gibel carp, including the leading variety clone A+, candidate variety clone F and wild clone H. Three clones showed distinct resistances to CaHV. Moreover, 8772, 8679 and 10,982 differentially expressed unigenes (DEUs) were identified from comparative transcriptomes between diseased individuals and control individuals of clone A+, F and H, respectively. Comprehensive analysis of the shared DEUs in all three clones displayed common defense pathways to the herpesvirus infection, activating IFN system and suppressing complements. KEGG pathway analysis of specifically changed DEUs in respective clones revealed distinct immune responses to the herpesvirus infection. The DEU numbers identified from clone H in KEGG immune-related pathways, such as "chemokine signaling pathway", " Toll-like receptor signaling pathway" and others, were remarkably much more than those from clone A+ and F. Several IFN-related genes, including Mx1, viperin, PKR and others, showed higher increases in the resistant clone H than that in the others. IFNphi3, IFI44-like and Gig2 displayed the highest expression in clone F and IRF1 uniquely increased in susceptible clone A+. In contrast to strong immune defense in resistant clone H, susceptible clone A+ showed remarkable up-regulation of genes related to apoptosis or death, indicating that clone A+ failed to resist virus offensive and evidently induced apoptosis or death. Conclusions: Our study is the first attempt to screen distinct resistances and immune responses of three gynogenetic gibel carp clones to herpesvirus infection by comprehensive transcriptomes. These differential DEUs, immune-related pathways and IFN system genes identified from susceptible and resistant clones will be beneficial to marker-assisted selection (MAS) breeding or molecular module-based resistance breeding in gibel carp. |
资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; earmarked fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01] |
WOS关键词 | CARASSIUS-AURATUS-GIBELIO ; TROUT ONCORHYNCHUS-MYKISS ; SILVER CRUCIAN CARP ; FLOUNDER PARALICHTHYS-OLIVACEUS ; GENE-EXPRESSION ; MOLECULAR CHARACTERIZATION ; TELEOST FISH ; HEMATOPOIETIC NECROSIS ; CAB CELLS ; FUNCTIONAL-CHARACTERIZATION |
WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
语种 | 英语 |
出版者 | BIOMED CENTRAL LTD |
WOS记录号 | WOS:000406619700005 |
资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; 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earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; 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Strategic Priority Research Program of the Chinese Academy of Sciences ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; earmarked fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; 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内容类型 | 期刊论文 |
源URL | [http://ir.ihb.ac.cn/handle/342005/31441] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Zhou, Li; Gui, Jian-Fang |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Gao, Fan-Xiang,Wang, Yang,Zhang, Qi-Ya,et al. Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes[J]. BMC GENOMICS,2017,18(1):19. |
APA | Gao, Fan-Xiang.,Wang, Yang.,Zhang, Qi-Ya.,Mou, Cheng-Yan.,Li, Zhi.,...&Gui, Jian-Fang.(2017).Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes.BMC GENOMICS,18(1),19. |
MLA | Gao, Fan-Xiang,et al."Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes".BMC GENOMICS 18.1(2017):19. |
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