Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis

doi:10.1261/rna.067090.118

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis
	He, Wen-Xia 1,2; Wu, Min 1,2; Liu, Zhen 1,2; Li, Zhi 1; Wang, Yang 1,2; Zhou, Jian 1,2; Yu, Peng 1,2; Zhang, Xiao-Juan 1; Zhou, Li 1,2; Gui, Jian-Fang 1,2
刊名	RNA
	2018-12-01
卷号	24 期号:12 页码:1738-1748
关键词	slbp2 replication-dependent histones ribosomal proteins maternal effect oogenesis early embryogenesis
ISSN号	1355-8382
DOI	10.1261/rna.067090.118
通讯作者	Zhou, Li(zhouli@ihb.ac.cn) ; Gui, Jian-Fang(jfgui@ihb.ac.cn)
英文摘要	Stem-loop binding protein (SLBP) is required for replication-dependent histone mRNA metabolism in mammals. Zebrafish possesses two slbps, and slbp1 is necessary for retinal neurogenesis. However, the detailed expression and function of slbp2 in zebrafish are still unknown. In this study, we first identified zebrafish slbp2 as an oocyte-specific maternal factor and then generated a maternal-zygotic slbp2F3 homozygous mutant (MZslbp2 Delta 4(-/-)) using CRISPR/Cas9. The depletion of maternal Slbp2 disrupted early nuclear cleavage, which resulted in developmental arrest at the MBT stage. The developmental defects could be rescued in slbp2transgenic MZslbp2 Delta 4(-/-) embryos. However, homozygous mutant MZslbp1 Delta 1(-/-)developed normally, indicating slbp1 is dispensable for zebrafish early embryogenesis. Through comparative proteome and transcriptome profiling between WT and MZslbp2 Delta 4(-/- )embryos, we identified many differentially expressed proteins and genes. In comparison with those in WT embryos, four replication-dependent histones, including H2a, H2b, H3, and H4, all reduced their expression, while histone variant h2afx significantly increased in MZslbp2 Delta 4(-/- )embryos at the 256-cell stage and high stage. Zebrafish Slbp2 can bind histone mRNA stem-loop in vitro, and the defects of MZslbp2 Delta 4(-/-) embryos can be partially rescued by overexpression of H2b. The current data indicate that maternal Slbp2 plays a pivotal role in the storage of replication-dependent histone mRNAs and proteins during zebrafish oogenesis.
资助项目	Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDY-SSW-SMC025] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; Earmarked Fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01]
WOS关键词	LOOP-BINDING-PROTEIN ; EARLY EMBRYONIC-DEVELOPMENT ; STEM-LOOP ; MESSENGER-RNA ; GENE-EXPRESSION ; H2AX PHOSPHORYLATION ; GENOMIC INSTABILITY ; POLY(A) TAIL ; IN-VIVO ; TRANSLATION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
WOS记录号	WOS:000450373800011
资助机构	Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Earmarked Fund for Modern Agro-industry Technology Research System ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/29021]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Zhou, Li; Gui, Jian-Fang
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He, Wen-Xia,Wu, Min,Liu, Zhen,et al. Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis[J]. RNA,2018,24(12):1738-1748.
APA	He, Wen-Xia.,Wu, Min.,Liu, Zhen.,Li, Zhi.,Wang, Yang.,...&Gui, Jian-Fang.(2018).Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis.RNA,24(12),1738-1748.
MLA	He, Wen-Xia,et al."Oocyte-specific maternal Slbp2 is required for replication-dependent histone storage and early nuclear cleavage in zebrafish oogenesis and embryogenesis".RNA 24.12(2018):1738-1748.