Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs

doi:10.1111/jfb.13875

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs
	Chen, Liangming 1,2; Huang, Rong 1; Zhu, Denghui 1,2; Yang, Cheng 1,2; He, Libo 1; Li, Yongming 1; Liao, Lanjie 1; Zhu, Zuoyan 1; Wang, Yaping 1
刊名	JOURNAL OF FISH BIOLOGY
	2019
卷号	94 期号:1 页码:132-141
关键词	deep sequencing dmrt grass carp microRNA sex differentiation
ISSN号	0022-1112
DOI	10.1111/jfb.13875
通讯作者	Wang, Yaping(wangyp.ihb@gmail.com)
英文摘要	This research identified 169 known microRNAs (miRNAs), 380 novel miRNAs, and 30,538 targets in 11 tissues (blood, brain, derma, gill, heart, intestine, kidney, liver, muscle, pronephros, and spleen) from grass carp Ctenopharyngodon idella with high-throughput sequencing (HTS). Transcripts per million (TPM) expression analysis detected 41 brain-enriched miRNAs (accounting for 61.19% of all tissue-enriched miRNAs). Real-time quantitative PCR (RT-qPCR) confirmed that 21 of 24 randomly selected tissue-enriched miRNAs from the TPM analysis were indeed tissue-enriched (P < 0.05), suggesting the HTS and TPM analyses were reliable. Nine of the 41 brain-enriched miRNAs are complementary to members of the double-sex and mab-3 related transcription factor family (dmrt) involved in sex differentiation. RT-qPCR revealed that cid-miR-138 was more highly expressed in testis than in ovary (P < 0.01), while the reverse was true for target gene dmrt4a (P < 0.01). This opposite expression pattern suggested the direct participation of cid-miR-138-dmrt4a in neuroendocrine mechanisms related to brain-pituitary networks during sex development. The discovery of miRNAs from 11 C. idella tissues expands the available fish miRNA database, and enhances our understanding of the role of sex-related miRNAs in tissue differentiation and maintenance of specific tissue functions in fishes.
资助项目	National Natural Science Foundation of China[31572614] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA08030203] ; State of Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ02]
WOS关键词	EXPRESSION ; GENES ; EVOLUTION ; IDENTIFICATION ; MECHANISMS ; INSIGHTS ; GENOME ; TARGET ; FISH ; KEGG
WOS研究方向	Fisheries ; Marine & Freshwater Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000457463200015
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology ; State of Key Laboratory of Freshwater Ecology and Biotechnology
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28625]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Wang, Yaping
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Chen, Liangming,Huang, Rong,Zhu, Denghui,et al. Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs[J]. JOURNAL OF FISH BIOLOGY,2019,94(1):132-141.
APA	Chen, Liangming.,Huang, Rong.,Zhu, Denghui.,Yang, Cheng.,He, Libo.,...&Wang, Yaping.(2019).Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs.JOURNAL OF FISH BIOLOGY,94(1),132-141.
MLA	Chen, Liangming,et al."Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs".JOURNAL OF FISH BIOLOGY 94.1(2019):132-141.