Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures

doi:10.1021/acsami.8b00246

CORC > 化学研究所 > 中国科学院化学研究所 > 胶体、界面与化学热力学实验室

	Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures
	Cao, Meiwen 1,2; Wang, Yu 1,2; Zhao, Wenjing 1,2; Qi, Ruilian 3; Han, Yuchun 3; Wu, Rongliang 4; Wang, Yilin 3; Xu, Hai 1,2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2018-07-25
卷号	10 期号:29 页码:24349-24360
关键词	Amphiphilic Peptide Dna Condensation Self-assembly Virus-mimicking Gene Delivery Molecular Simulation
ISSN号	1944-8244
DOI	10.1021/acsami.8b00246
英文摘要	A series of surfactant-like peptides have been designed for inducing DNA condensation, which are all comprised of the same set of amino acids in different sequences. Results from experiments and molecular dynamics simulations show that the peptide's self-assembly and DNA-interaction behaviors can be well manipulated through sequence variation. With optimized pairing modes between the beta-sheets, the peptide of I(3)V(3)A(3)G(3)K(3) can induce efficient DNA condensation into virus-mimicking structures. The condensation involves two steps; the peptide molecules first bind onto the DNA chain through electrostatic interactions and then self-associate into beta-sheets under hydrophobic interactions and hydrogen bonding. In such condensates, the peptide beta-sheets act as scaffolds to assist the ordered arrangement of DNA, mimicking the very nature of the virus capsid in helping DNA packaging. Such a hierarchy affords an extremely stable structure to attain the highly condensed state and protect DNA against enzymatic degradation. Moreover, the condensate size can be well tuned by the DNA length. The condensates with smaller sizes and narrow size distribution can deliver DNA efficiently into cells. The study helps not only for probing into the DNA packaging mechanism in virus but also delineating the role of peptide self-assembly in DNA condensation, which may lead to development of peptide-based gene vectors for therapeutic applications.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000440511900005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/41942]
专题	化学研究所_胶体、界面与化学热力学实验室
通讯作者	Cao, Meiwen; Wu, Rongliang; Xu, Hai
作者单位	1.China Univ Petr East China, Coll Chem Engn, State Key Lab Heavy Oil Proc, 66 Changjiang West Rd, Qingdao 266580, Peoples R China 2.China Univ Petr East China, Coll Chem Engn, Ctr Bioengn & Biotechnol, 66 Changjiang West Rd, Qingdao 266580, Peoples R China 3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Colloid & Interface Sci, Beijing 100190, Peoples R China 4.Donghua Univ, Coll Mat Sci & Engn, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China
推荐引用方式 GB/T 7714	Cao, Meiwen,Wang, Yu,Zhao, Wenjing,et al. Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(29):24349-24360.
APA	Cao, Meiwen.,Wang, Yu.,Zhao, Wenjing.,Qi, Ruilian.,Han, Yuchun.,...&Xu, Hai.(2018).Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures.ACS APPLIED MATERIALS & INTERFACES,10(29),24349-24360.
MLA	Cao, Meiwen,et al."Peptide-Induced DNA Condensation into Virus-Mimicking Nanostructures".ACS APPLIED MATERIALS & INTERFACES 10.29(2018):24349-24360.