Unmatched masses in peptide mass fingerprints caused by cross-contamination: An updated statistical result

doi:10.1002/pmic.200300452

CORC > 化学研究所 > 中国科学院化学研究所

	Unmatched masses in peptide mass fingerprints caused by cross-contamination: An updated statistical result
	Ding, QX ; Xiao, L ; Xiong, SX ; Jia, YF ; Que, HP ; Guo, YJ ; Liu, SJ
刊名	PROTEOMICS
	2003-07-01
卷号	3 期号:7 页码:1313-1317
关键词	Cross Contamination Keratins Peptide Mass Fingerprint
ISSN号	1615-9853
DOI	10.1002/pmic.200300452
英文摘要	Unmatched masses are often observed in the experimental peptide mass spectra when database searching is performed with the ProFound program. Comparison between theoretical and experimental mass spectra of standard proteins shows that contamination accounts for most of the unmatched masses. In this retrospective analysis, the top 100 most probable contaminating masses, as listed in order of their probability, are statistically filtered out from 118 different experimental peptide mass fingerprinting (PMF) maps. Most of the interfering masses originate from trypsin autolysis and human keratins. Subtraction of known contaminants from raw data and using cleaner masses for searching can enhance protein identification by PMF
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000184507200023
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/80929]
专题	中国科学院化学研究所
通讯作者	Liu, SJ
作者单位	1.Chinese Acad Med Sci, Inst Basic Med Sci, Dept Neurobiol, Beijing 100850, Peoples R China 2.Beijing Mass Spectronomy Ctr, Inst Chem, Beijing, Peoples R China 3.Inst Biophys, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ding, QX,Xiao, L,Xiong, SX,et al. Unmatched masses in peptide mass fingerprints caused by cross-contamination: An updated statistical result[J]. PROTEOMICS,2003,3(7):1313-1317.
APA	Ding, QX.,Xiao, L.,Xiong, SX.,Jia, YF.,Que, HP.,...&Liu, SJ.(2003).Unmatched masses in peptide mass fingerprints caused by cross-contamination: An updated statistical result.PROTEOMICS,3(7),1313-1317.
MLA	Ding, QX,et al."Unmatched masses in peptide mass fingerprints caused by cross-contamination: An updated statistical result".PROTEOMICS 3.7(2003):1313-1317.