Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge

CORC > 化学研究所 > 中国科学院化学研究所

	Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge
	Li, JR ; Rosilio, V ; Boissonnade, MM ; Baszkin, A
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2003-05-15
卷号	29 期号:1 页码:13-20
关键词	Protein Adsorption Surface Tension Enzyme Glucose Oxidase Phospholipid Gox Penetration
ISSN号	0927-7765
英文摘要	The effect of both lipid headgroup charge and of aqueous subphase pH on adsorption of glucose oxidase molecules (GOx) into dibehenoylphosphatidylcholine (DBPC) and octadecylamine (ODA) monolayers has been studied by surface tension and surface pressure measurements. The results revealed that whereas the extent of GOx adsorption into pure DBPC monolayers essentially depended on the area in a monolayer available to harbor adsorbing GOx molecules, enhanced enzyme penetration into a monolayer occurred at high surface densities. The kinetics of enzyme penetration was found to be dependent of the initial surface pressure of a spread monolayer. The addition of ODA to DBPC monolayers gave a dramatic boost to GOx penetration and at high molecular surface densities largely depended on the ratio of ODA in the mixed monolayers. This increase in GOx penetration was attributed to the strong attractive electrostatic interaction between the positively charged ODA and the negatively charged enzyme. (C) 2002 Elsevier Science B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000182777100002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/80047]
专题	中国科学院化学研究所
通讯作者	Rosilio, V
作者单位	1.Lab Physicochim Surface, CNRS, UMR 8612, F-92296 Chatenay Malabry, France 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Li, JR,Rosilio, V,Boissonnade, MM,et al. Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2003,29(1):13-20.
APA	Li, JR,Rosilio, V,Boissonnade, MM,&Baszkin, A.(2003).Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge.COLLOIDS AND SURFACES B-BIOINTERFACES,29(1),13-20.
MLA	Li, JR,et al."Adsorption of glucose oxidase into lipid monolayers: effect of a lipid headgroup charge".COLLOIDS AND SURFACES B-BIOINTERFACES 29.1(2003):13-20.