pH value and ionic strength effects on the adsorption kinetics of protein/phospholipid at the chloroform/water interface

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	pH value and ionic strength effects on the adsorption kinetics of protein/phospholipid at the chloroform/water interface
	Yan, LL ; Zhang, Y ; Cui, GC ; Li, JB
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2000-12-15
卷号	175 期号:1-2 页码:61-66
关键词	Beta-lactoglobulin Chloroform/water Interface Axisymmetric Drop Shape Analysis
ISSN号	0927-7757
英文摘要	The adsorption kinetics of beta-lactoglobulin in an aqueous buffer solution in the presence of phospholipid, L-alpha-dipalmitoylphosphatidylethanolamine (DPPE), dissolved in chloroform, has been investigated by using a pendent drop technique. The dynamic interfacial tension for the mixed beta-lactoglobulin/phospholipid layer was measured as a function of time at a constant phospholipid concentration, C-DPPE = I x 10(-6) M. Experimentally it has been found that increase of ionic strength of aqueous solution can enhance the adsorption rate of the protein. However, the larger pH value leads to the adsorption process being reduced. When the protein concentration was increased up to 3.8 mg l(-1), the effect of the salt concentration on the adsorption can be neglected and the protein dominates principally the adsorption process. In the acid aqueous solution, pH 5, approaching equilibrium time of adsorption for protein was around 1300 s and twice longer than in the base subphase, pH 8. However, the equilibrium interfacial tension was independent on the pH value. The salt concentration and pH value effects show that both the electrostatic interaction and hydrophobic effect make the mixed lipid/beta-lactoglobulin layer stable at the liquid/liquid interface. (C) 2000 Published by Elsevier Science B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000089562300008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/76143]
专题	中国科学院化学研究所
通讯作者	Li, JB
作者单位	1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Inst Photog Chem,Int Joint Lab, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Max Planck Inst Colloid & Interface Sci, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Yan, LL,Zhang, Y,Cui, GC,et al. pH value and ionic strength effects on the adsorption kinetics of protein/phospholipid at the chloroform/water interface[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2000,175(1-2):61-66.
APA	Yan, LL,Zhang, Y,Cui, GC,&Li, JB.(2000).pH value and ionic strength effects on the adsorption kinetics of protein/phospholipid at the chloroform/water interface.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,175(1-2),61-66.
MLA	Yan, LL,et al."pH value and ionic strength effects on the adsorption kinetics of protein/phospholipid at the chloroform/water interface".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 175.1-2(2000):61-66.