Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens

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	Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens
	Li, XH ; Deng, XM ; Yuan, ML ; Xiong, CD ; Huang, ZT ; Zhang, YH ; Jia, WX
刊名	INTERNATIONAL JOURNAL OF PHARMACEUTICS
	1999-02-15
卷号	178 期号:2 页码:245-255
关键词	Poly-dl-lactide-poly(Ethylene Glycol) Outer Membrane Protein Preparative Parameter Encapsulation Efficiency In Vitro Release
ISSN号	0378-5173
英文摘要	Block copolymer, poly-DL-lactide-poly(ethylene glycol) (PELA) with 11.5% of poly(ethylene glycol) (PEG) content was prepared by bulk ring-opening polymerization using stannous chloride as initiator. PELA microspheres with entrapped Leptospira Interrogans antigens, outer membrane protein (OMP) were elaborated by solvent extraction method based on the formation of multiple w/o/w emulsion, and the resulting microspheres were characterized with respect to particle size, OMP entrapment and morphology characteristics. The purpose of the present study is to perform the optimization of preparative parameters for OMP-loaded PELA micropsheres to control particle size and improve the OMP encapsulation efficiency. Of all the parameters investigated, the polymer concentration of organic phase and the external aqueous phase volume play major roles on particle size, while the organic phase volume, internal aqueous phase volume and the addition of surfactant into the internal aqueous phase display considerable effects on OMP loading efficiency. A small volume of internal aqueous phase and intermediate volumes of organic phase and external aqueous phase were favorable to achieve micropsheres with a size of 1-2 mu m and high antigen encapsulation efficiency (70-80%). In vitro OMP release profiles from PELA microspheres consist of a small burst release followed by a gradual release phase. The OMP release rate shows some relations with the porous and water-swollen inner structure of the microspheres matrix. The presence of surfactant in microspheres accelerates OMP release, but the OMP entrapment within microspheres shows limited effects on the release profile. (C) 1999 Elsevier Science B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000078849000010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/75109]
专题	中国科学院化学研究所
通讯作者	Li, XH
作者单位	1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.W China Univ Med Sci, Chengdu 610041, Peoples R China
推荐引用方式 GB/T 7714	Li, XH,Deng, XM,Yuan, ML,et al. Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,1999,178(2):245-255.
APA	Li, XH.,Deng, XM.,Yuan, ML.,Xiong, CD.,Huang, ZT.,...&Jia, WX.(1999).Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens.INTERNATIONAL JOURNAL OF PHARMACEUTICS,178(2),245-255.
MLA	Li, XH,et al."Investigation on process parameters involved in preparation of poly-DL-lactide-poly(ethylene glycol) microspheres containing Leptospira Interrogans antigens".INTERNATIONAL JOURNAL OF PHARMACEUTICS 178.2(1999):245-255.