Formation and characteristics of an unusual lambda-DNA species

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	Formation and characteristics of an unusual lambda-DNA species
	Cao, EH ; Sun, XG ; Bai, CL ; Liu, CQ ; He, YJ ; Wang, XW ; Fang, Y
刊名	BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
	1997-10-01
卷号	43 期号:3 页码:541-550
关键词	Lambda-dna Triple-stranded Dna Melting Temperature Cd Spectra Fluorescence Spectrum Transmission Electron Microscope Atomic Force Microscope
ISSN号	1039-9712
英文摘要	An unusual DNA species, termed as DNA species A, has been isolated and purified from thermal-denatured lambda-DNA Hind III by Sephadex G-200 gel filtration. Our studies indicate that DNA species A is resistant to DNase I digestion and has a higher melting point. The new DNA species showed a lower absorbency at 260 nm, and a lower fluorescence quantum yield after interaction with ethidium bromide (EB) than native double-stranded lambda-DNA. CD spectrum of DNA species A consists of a broad positive band centered at 245 nm and a weak negative band at 220 nm. transmission electron microscope (TEM) visualizations showed that their lengths of DNA species A fell mainly in three regions (300-500 nm, 750-1000 nm and 1500 nm) that corresponded to three fluorescence bands in the EB-stained gels. Their apparent width and height were 65-75 nm and 2.2 nm respectively as observed by images of atomic force microscope (AFM).
语种	英语
出版者	ACADEMIC PRESS AUST
WOS记录号	WOS:000073191000009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/74159]
专题	中国科学院化学研究所
通讯作者	Cao, EH
作者单位	1.Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China 2.Acad Sinica, Inst Chem, Beijing 100080, Peoples R China 3.Acad Sinica, Inst Zool, Kunming 650223, Peoples R China
推荐引用方式 GB/T 7714	Cao, EH,Sun, XG,Bai, CL,et al. Formation and characteristics of an unusual lambda-DNA species[J]. BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,1997,43(3):541-550.
APA	Cao, EH.,Sun, XG.,Bai, CL.,Liu, CQ.,He, YJ.,...&Fang, Y.(1997).Formation and characteristics of an unusual lambda-DNA species.BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,43(3),541-550.
MLA	Cao, EH,et al."Formation and characteristics of an unusual lambda-DNA species".BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 43.3(1997):541-550.