Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase

doi:10.1002/adfm.201100216

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	Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase
	He, Fang 1; Liu, Libing 2; Li, Lidong 1
刊名	ADVANCED FUNCTIONAL MATERIALS
	2011-08-23
卷号	21 期号:16 页码:3143-3149
ISSN号	1616-301X
DOI	10.1002/adfm.201100216
英文摘要	A series of water-soluble polyfluorene derivatives containing diketopyrrolopyrrole derivative units are synthesized and characterized. These copolymers, poly[9,9'-bis(6 ''-N,N,N-trimethyl ammonium) hexylfluorene-co-alt-2,5-bis (6 ''-N,N,N-trimethylammonium) hexylpyrrolo[3,4-c]pyrrole-1,4(2H, 5H)-dione] (PFDPN), demonstrate intramolecular energy transfer from the fluorene units to the diketopyrrolopyrrole derivative units, and show red-shifted emissions in aqueous solution. The PFDPN polymers can combine with Cy5-labeled ssDNA by electrostatic interactions and efficiently amplify the fluorescence signal of red Cy5 dye through fluorescence resonance energy transfer. Moreover, based on DNA replacement method, this amplification system can be used to monitor the template-independent DNA elongation process catalyzed by terminal deoxynucleotidyl transferase.
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000294166200016
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/71277]
专题	中国科学院化学研究所
通讯作者	He, Fang
作者单位	1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	He, Fang,Liu, Libing,Li, Lidong. Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase[J]. ADVANCED FUNCTIONAL MATERIALS,2011,21(16):3143-3149.
APA	He, Fang,Liu, Libing,&Li, Lidong.(2011).Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase.ADVANCED FUNCTIONAL MATERIALS,21(16),3143-3149.
MLA	He, Fang,et al."Water-Soluble Conjugated Polymers for Amplified Fluorescence Detection of Template-Independent DNA Elongation Catalyzed by Polymerase".ADVANCED FUNCTIONAL MATERIALS 21.16(2011):3143-3149.