Silver Ions-Mediated Conformational Switch: Facile Design of Structure-Controllable Nucleic Acid Probes

doi:10.1021/ac101114w

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	Silver Ions-Mediated Conformational Switch: Facile Design of Structure-Controllable Nucleic Acid Probes
	Wang, Yongxiang 1,2; Li, Jishan 1; Wang, Hao 1,2; Jin, Jianyu 1,2; Liu, Jinhua 2; Wang, Kemin 1; Tan, Weihong 1,3,4,5; Yang, Ronghua 1
刊名	ANALYTICAL CHEMISTRY
	2010-08-01
卷号	82 期号:15 页码:6607-6612
ISSN号	0003-2700
DOI	10.1021/ac101114w
英文摘要	Conformationally constraint nucleic acid probes were usually designed by forming an intramolecular duplex based on Watson-Crick hydrogen bonds. The disadvantages of these approaches are the inflexibility and instability in complex environment of the Watson-Crick-based duplex. We report that this hydrogen bonding pattern can be replaced by metal-ligation between specific metal ions and the natural bases. To demonstrate the feasibility of this principle, two linear oligonucleotides and silver ions were examined as models for DNA hybridization assay and adenosine triphosphate detection. The both nucleic acids contain target binding sequences in the middle and cytosine (C)-rich sequences at the lateral portions. The strong interaction between Ag(+) ions and cytosines forms stable C-Ag(+)-C structures, which promises the oligonucleotides to form conformationally constraint formations. In the presence of its target, interaction between the loop sequences and the target unfolds the C-Ag(+)-C structures, and the corresponding probes unfolding can be detected by a change in their fluorescence emission. We discuss the thermodynamic and kinetic opportunities that are provided by using Ag(+) ion complexes instead of traditional Watson-Crick-based duplex. In particular, the intrinsic feature of the metal-ligation motif facilitates the design of functional nucleic acids probes by independently varying the concentration of Ag(+) ions in the medium.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000280401400042
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/69805]
专题	中国科学院化学研究所
通讯作者	Yang, Ronghua
作者单位	1.Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 3.Univ Florida, Ctr Res Bionano Interface, Dept Chem, Gainesville, FL 32611 USA 4.Univ Florida, Dept Physiol & Funct Genom, Shands Canc Ctr, Gainesville, FL 32611 USA 5.Univ Florida, UF Genet Inst, Gainesville, FL 32611 USA
推荐引用方式 GB/T 7714	Wang, Yongxiang,Li, Jishan,Wang, Hao,et al. Silver Ions-Mediated Conformational Switch: Facile Design of Structure-Controllable Nucleic Acid Probes[J]. ANALYTICAL CHEMISTRY,2010,82(15):6607-6612.
APA	Wang, Yongxiang.,Li, Jishan.,Wang, Hao.,Jin, Jianyu.,Liu, Jinhua.,...&Yang, Ronghua.(2010).Silver Ions-Mediated Conformational Switch: Facile Design of Structure-Controllable Nucleic Acid Probes.ANALYTICAL CHEMISTRY,82(15),6607-6612.
MLA	Wang, Yongxiang,et al."Silver Ions-Mediated Conformational Switch: Facile Design of Structure-Controllable Nucleic Acid Probes".ANALYTICAL CHEMISTRY 82.15(2010):6607-6612.