Substitution of chloride by bromide modifies the low-temperature tyrosine Z oxidation in active photosystem II

doi:10.1016/j.bbabio.2010.02.025

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	Substitution of chloride by bromide modifies the low-temperature tyrosine Z oxidation in active photosystem II
	Ren, Yanan; Zhang, Chunxi; Zhao, Jingquan
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
	2010-08-01
卷号	1797 期号:8 页码:1421-1427
关键词	Photosystem Ii Tyrosine z Mn-cluster Chloride Bromide Substitution Electron Paramagnetic Resonance
ISSN号	0005-2728
DOI	10.1016/j.bbabio.2010.02.025
英文摘要	Chloride is an essential cofactor for photosynthetic water oxidation. However, its location and functional roles in active photosystem II are still a matter of debate. We have investigated this issue by studying the effects of Cl(-) replacement by Br(-) in active PSII. In Br(-) substituted samples, Cl(-) is effectively replaced by Br(-) in the presence of 1.2 M NaBr under room light with protection of anaerobic atmosphere followed by dialysis. The following results have been obtained. i) The oxygen-evolving activities of the Br(-)-PSII samples are significantly lower than that of the Cl(-)-PSII samples; ii) The same S(2) multiline EPR signals are observed in both Br(-) and Cl(-)-PSII samples; iii) The amplitudes of the visible light induced S(1)Tyr(z)(center dot) and S(2)Tyr(z)(center dot) EPR signals are significantly decreased after Br(-) substitution; the S(1)Tyr(z)(center dot) EPR signal is up-shifted about 8 G. whereas the S(2)Tyr(z)(center dot) signal is down-shifted about 12 G after Br(-) substitution. These results imply that the redox properties of Tyr(z) and spin interactions between Tyr(z)(center dot) and Mn-cluster could be significantly modified due to Br(-) substitution. It is suggested that Cl(-)/Br(-) probably coordinates to the Ca(2+) ion of the Mn-cluster in active photosystem II. (C) 2010 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000279888700008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/69595]
专题	中国科学院化学研究所
通讯作者	Zhang, Chunxi
作者单位	Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Lab Photochem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Ren, Yanan,Zhang, Chunxi,Zhao, Jingquan. Substitution of chloride by bromide modifies the low-temperature tyrosine Z oxidation in active photosystem II[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2010,1797(8):1421-1427.
APA	Ren, Yanan,Zhang, Chunxi,&Zhao, Jingquan.(2010).Substitution of chloride by bromide modifies the low-temperature tyrosine Z oxidation in active photosystem II.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1797(8),1421-1427.
MLA	Ren, Yanan,et al."Substitution of chloride by bromide modifies the low-temperature tyrosine Z oxidation in active photosystem II".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1797.8(2010):1421-1427.