High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy | |
Sang, Min1,5; Ma, Fei2; Xie, Jie3; Chen, Xiao-Bo4; Wang, Ke-Bin1; Qin, Xiao-Chun1; Wang, Wen-Da1; Zhao, Jing-Quan3; Li, Liang-Bi1; Zhang, Jian-Ping2 | |
刊名 | BIOPHYSICAL CHEMISTRY |
2010 | |
卷号 | 146期号:1页码:7-12 |
关键词 | Chlorophyll a Cyt b(6)f Singlet Oxygen Photo-bleaching Epr |
ISSN号 | 0301-4622 |
DOI | 10.1016/j.bpc.2009.09.012 |
英文摘要 | Electron paramagnetic resonance (EPR) spectroscopy was used to detect the light-induced formation of singlet oxygen (O-1(2)center dot) in the intact and the Rieske-depleted cytochrome b(6)f complexes (Cyt b(6)f) from Bryopsis corticulans, as well as in the isolated Rieske Fe-S protein. It is shown that, under white-light illumination and aerobic conditions, chlorophyll a (Chl a) bound in the intact Cyt b(6)f can be bleached by light-induced O-1(2)center dot, and that the 10; production can be promoted by D2O or scavenged by extraneous antioxidants such as L-histidine, ascorbate, beta-carotene and glutathione. Under similar experimental conditions, O-1(2)center dot was also detected in the Rieske-depleted Cyt b(6)f complex, but not in the isolated Rieske Fe-S protein. The results prove that Chl a cofactor, rather than Rieske Fe-S protein, is the specific site of O-1(2)center dot formation, a conclusion which draws further support from the generation of O-1(2)center dot with selective excitation of Chl a using monocolor red light. (C) 2009 Elsevier B.V. All rights reserved. |
语种 | 英语 |
出版者 | ELSEVIER SCIENCE BV |
WOS记录号 | WOS:000272440400002 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/69051] |
专题 | 中国科学院化学研究所 |
通讯作者 | Li, Liang-Bi |
作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China 4.Hebei Univ Sci & Technol, Sch Biosci & Engn, Shijiazhuang 050018, Hebei Province, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Sang, Min,Ma, Fei,Xie, Jie,et al. High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy[J]. BIOPHYSICAL CHEMISTRY,2010,146(1):7-12. |
APA | Sang, Min.,Ma, Fei.,Xie, Jie.,Chen, Xiao-Bo.,Wang, Ke-Bin.,...&Kuang, Ting-Yun.(2010).High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy.BIOPHYSICAL CHEMISTRY,146(1),7-12. |
MLA | Sang, Min,et al."High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy".BIOPHYSICAL CHEMISTRY 146.1(2010):7-12. |
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