Label-free and sequence-specific DNA detection down to a picomolar level with carbon nanotubes as support for probe DNA

doi:10.1016/j.aca.2009.05.017

CORC > 化学研究所 > 中国科学院化学研究所

	Label-free and sequence-specific DNA detection down to a picomolar level with carbon nanotubes as support for probe DNA
	Zhu, Ningning 1,2; Lin, Yuqing 1; Yu, Ping 1; Su, Lei 1; Mao, Lanqun 1
刊名	ANALYTICA CHIMICA ACTA
	2009-09-14
卷号	650 期号:1 页码:44-48
关键词	Single-walled Carbon Nanotubes Electrochemical Impedance Spectroscopy Sequence-specific Dna Detection
ISSN号	0003-2670
DOI	10.1016/j.aca.2009.05.017
英文摘要	This study describes a simple and label-free electrochemical impedance spectroscopic (EIS) method for sequence-specific detection of DNA by using single-walled carbon nanotubes (SWNTs) as the support for probe DNA. SWNTs are confined onto gold electrodes with mixed self-assembly monolayers of thioethanol and cysteamine. Single-stranded DNA (ssDNA) probe is anchored onto the SWNT support through covalent binding between carboxyl groups at the nanotubes and amino groups at Tends of ssDNA. Hybridization of target DNA with the anchored probe DNA greatly increases the interfacial electron-transfer resistance (R-et) at the double-stranded DNA(dsDNA)-modified electrodes for the redox couple of Fe(CN)(6)(3-/4-), which could be used for label-free and sequence-specific DNA detection. EIS results demonstrate that the utilization of SWNTs as the support for probe DNA substantially increases the surface loading of probe DNA onto electrode surface and thus remarkably lowers the detection limit for target DNA. Under the conditions employed here, R-et is linear with the concentration of target DNA within a concentration range from 1 to 10 pM with a detection limit down to 0.8 pM (S/N = 3). This study may offer a novel and label-free electrochemical approach to sensitive sequence-specific DNA detection. (C) 2009 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000270003800008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/68189]
专题	中国科学院化学研究所
通讯作者	Mao, Lanqun
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Shanghai Normal Univ, Dept Chem, Coll Life & Environm Sci, Shanghai 200234, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Ningning,Lin, Yuqing,Yu, Ping,et al. Label-free and sequence-specific DNA detection down to a picomolar level with carbon nanotubes as support for probe DNA[J]. ANALYTICA CHIMICA ACTA,2009,650(1):44-48.
APA	Zhu, Ningning,Lin, Yuqing,Yu, Ping,Su, Lei,&Mao, Lanqun.(2009).Label-free and sequence-specific DNA detection down to a picomolar level with carbon nanotubes as support for probe DNA.ANALYTICA CHIMICA ACTA,650(1),44-48.
MLA	Zhu, Ningning,et al."Label-free and sequence-specific DNA detection down to a picomolar level with carbon nanotubes as support for probe DNA".ANALYTICA CHIMICA ACTA 650.1(2009):44-48.