Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance

doi:10.1016/j.bios.2009.06.020

CORC > 化学研究所 > 中国科学院化学研究所

	Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance
	Ma, Wei 1; Chen, Wei 1; Qiao, Ruirui 2; Liu, Chunyan 2; Yang, Chunhui 2; Li, Zhuokun 1; Xu, Dinghua 1; Peng, Chifang 1; Jin, Zhengyu 1; Xu, Chuanlai 1
刊名	BIOSENSORS & BIOELECTRONICS
	2009-09-15
卷号	25 期号:1 页码:240-243
关键词	Detection Microcystin Immunosensor Nuclear Magnetic Resonance Sensitive Magnetic Nanoparticles
ISSN号	0956-5663
DOI	10.1016/j.bios.2009.06.020
英文摘要	A stable and sensitive toxin residues immunosensor based on the relaxation of magnetic nanoparticles was developed. The method was performed in one reaction and offered sensitive, fast detection of target toxin residues in water. The target analyte, microcystin-LR (MC-LR) in Tai lake water, competed with the antigens on the surface of the magnetic nanoparticles and then influenced the formation of aggregates of the magnetic nanoparticles. Accordingly, the magnetic relaxation time of the magnetic nanoparticles was changed under the effect of the target analyte. The calibration curve was deduced at different concentrations of the target analyte. The limit of detection (LOD) of MC-LR was 0.6 ng g(-1) and the detection range was 1-18 ng g(-1). Another important feature of the developed method was the easy operation: only two steps were needed (1) to mix the magnetic nanoparticle solution with the sample solution and (2) read the results through the instrument. Therefore, the developed method may be a useful tool for toxin residues sensing and may find widespread applications. (C) 2009 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000270538400039
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/67883]
专题	中国科学院化学研究所
通讯作者	Xu, Chuanlai
作者单位	1.Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu Prov, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 10008, Peoples R China
推荐引用方式 GB/T 7714	Ma, Wei,Chen, Wei,Qiao, Ruirui,et al. Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance[J]. BIOSENSORS & BIOELECTRONICS,2009,25(1):240-243.
APA	Ma, Wei.,Chen, Wei.,Qiao, Ruirui.,Liu, Chunyan.,Yang, Chunhui.,...&Wang, Libing.(2009).Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance.BIOSENSORS & BIOELECTRONICS,25(1),240-243.
MLA	Ma, Wei,et al."Rapid and sensitive detection of microcystin by immunosensor based on nuclear magnetic resonance".BIOSENSORS & BIOELECTRONICS 25.1(2009):240-243.