Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion | |
Lin, Yuqing; Zhu, Ningning; Yu, Ping; Su, Lei; Mao, Lanqun | |
刊名 | ANALYTICAL CHEMISTRY |
2009-03-15 | |
卷号 | 81期号:6页码:2067-2074 |
ISSN号 | 0003-2700 |
DOI | 10.1021/ac801946s |
英文摘要 | This study demonstrates a new electroanalytical method with a high physiological relevance for simultaneous online monitoring of glucose and lactate in the striatum of the rat brain following global cerebral ischemia/reperfusion. The online analytical method is based on the efficient integration of in vivo microdialysis sampling with an online selective electrochemical detection with the electrochemical biosensors with dehydrogenases, i.e., glucose and lactate dehydrogenases, as recognition elements. The dehydrogenase-based electrochemical biosensors are developed onto the dual split-disk plastic carbon film (SPCF) electrodes with methylene green (MG) adsorbed onto single-walled carbon nanotubes (SWNTs) as the electrocatalyst for the oxidation of dihydronicotiamide adenine dinucleotide (NADH) at a low potential of 0.0 V (vs Ag/AgCl). Artificial cerebrospinal fluid (aCSF) containing NAD(+) is externally perfused from a second pump and online mixed with the brain microdialysates to minimize the variation of pH that occurred following the cerebral ischemia/reperfusion and to supply NAD(+) cofactor and O(2) for the enzymatic reactions of dehydrogenases and ascorbate oxidase, respectively. As a result, the developed online electroanalytical method exhibits a high selectivity against the electrochemically active species endogenously existing in the cerebral systems and a high tolerance against the variation of pH and O(2) following cerebral ischemia/reperfusion. This property, along with the good linearity and a high stability toward glucose and lactate as well as little cross-talk between two biosensors, substantially makes this method possible for the continuous, simultaneous, and online monitoring of glucose and lactate in the rat brain following global cerebral ischemia/reperfusion. This study establishes a new and effective platform for the investigation of the energy metabolism in physiological and pathological processes. |
语种 | 英语 |
出版者 | AMER CHEMICAL SOC |
WOS记录号 | WOS:000264142000005 |
内容类型 | 期刊论文 |
源URL | [http://ir.iccas.ac.cn/handle/121111/67649] |
专题 | 中国科学院化学研究所 |
通讯作者 | Mao, Lanqun |
作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Lin, Yuqing,Zhu, Ningning,Yu, Ping,et al. Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion[J]. ANALYTICAL CHEMISTRY,2009,81(6):2067-2074. |
APA | Lin, Yuqing,Zhu, Ningning,Yu, Ping,Su, Lei,&Mao, Lanqun.(2009).Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion.ANALYTICAL CHEMISTRY,81(6),2067-2074. |
MLA | Lin, Yuqing,et al."Physiologically Relevant Online Electrochemical Method for Continuous and Simultaneous Monitoring of Striatum Glucose and Lactate Following Global Cerebral Ischemia/Reperfusion".ANALYTICAL CHEMISTRY 81.6(2009):2067-2074. |
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