Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c

doi:10.1007/s11426-009-0067-2

CORC > 化学研究所 > 中国科学院化学研究所

	Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c
	Qin ChuanJiang; Tong Hui; Wang LiXiang
刊名	SCIENCE IN CHINA SERIES B-CHEMISTRY
	2009-06-01
卷号	52 期号:6 页码:833-839
关键词	Conjugated Polymer Fluorescence Sensor Water-soluble Side Chain
ISSN号	1006-9291
DOI	10.1007/s11426-009-0067-2
英文摘要	An anionic water-soluble polyfluorene derivative, poly(9,9-bis(6'-phosphatehexyl)fluorene-alt-1,4-phenylene) sodium salt (PFHPNa), was synthesized by Suzuki coupling reaction in DMF/water. Polymer PFHPNa was well soluble in water with a strong blue fluorescence emission. Effect of the side chain length on fluorescence sensory properties was studied by comparing quenching efficiencies toward different quenchers of PFHPNa with a reported polymer poly(9,9-bis(3'-phosphatepropyl)fluorene-alt-1,4-phenylene) sodium salt (PFPPNa), which have different side chains in length. For small molecular quenchers (methylviologen, MV(2+)) and meso-5,10,15,20-tetrakis-(N-methyl-4-pyridyl)porphine (TMPyP4), polymer PFHPNa had lower sensitivity due to the much longer side chain length. The positively charged metalloprotein cytochrome c could quench fluorescence of conjugated polymers via energy transfer and electron transfer. Moreover, polymer PFHPNa showed higher fluorescence quenching toward large biomolecules than PFPPNa. The corresponding Stern-Volmer (K (sv)) value of polymer PFHPNa was determined to be 2.1x10(8) M(-1) for cytochrome c. It could be used as a sensitive and selective fluorescence sensor for protein cytochrome c.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000266168000021
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/67067]
专题	中国科学院化学研究所
通讯作者	Wang LiXiang
作者单位	Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Qin ChuanJiang,Tong Hui,Wang LiXiang. Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2009,52(6):833-839.
APA	Qin ChuanJiang,Tong Hui,&Wang LiXiang.(2009).Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c.SCIENCE IN CHINA SERIES B-CHEMISTRY,52(6),833-839.
MLA	Qin ChuanJiang,et al."Water-soluble phosphate-functionalized polyfluorene as fluorescence biosensors toward cytochrome c".SCIENCE IN CHINA SERIES B-CHEMISTRY 52.6(2009):833-839.