Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces

doi:10.1002/polb.21880

CORC > 化学研究所 > 中国科学院化学研究所

	Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces
	Jing, Benxin 1,2; Yang, Qingbo 1; Zhu, Yingxi 3; Zhao, Jiang 1
刊名	JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS
	2009-12-15
卷号	47 期号:24 页码:2541-2546
关键词	Fluorescence Interfaces Polyelectrolytes
ISSN号	0887-6266
DOI	10.1002/polb.21880
英文摘要	The motion of DNA chain under electric field when it is in transient contact with the solid surfaces in aqueous Solution was studied by single molecule fluorescence microscopy at the total internal reflection geometry (TIRFM). In situ observation discovered that single lambda-DNA chains driven by electric field made transient contact with the solid sur-face and made hitting-sliding-leaving-like motion along the surface. By varying the surface chemistry from the negative-charged silanol group-rich surface to positive-charged amino group-rich surface, as well as hydrophobic surfaces, the dependence of DNA mobility on the surface-DNA inter-action was studied. The results show that a dependence of the mobility of DNA oil the surface polarity with respect to DNA itself. The study on different surfaces rich of silanol, amide, amino, and methyl groups show a sequence of DNA mobility of silanol > amide > amino. The mobility of DNA on methyl terminated surface was found to be similar to that on amino surface. (C) 2009 Wiley Periodicals, Inc J Polym Sci Part B Polym Phys 47 2541-2546, 2009
语种	英语
出版者	JOHN WILEY & SONS INC
WOS记录号	WOS:000272561100013
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66387]
专题	中国科学院化学研究所
通讯作者	Zhao, Jiang
作者单位	1.Chinese Acad Sci, Joint Lab Polymer Sci & Mat, State Key Lab Polymer Phys & Chem, Beijing Natl Lab Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100190, Peoples R China 3.Univ Notre Dame, Dept Chem & Biomol Engn, Notre Dame, IN 46556 USA
推荐引用方式 GB/T 7714	Jing, Benxin,Yang, Qingbo,Zhu, Yingxi,et al. Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces[J]. JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS,2009,47(24):2541-2546.
APA	Jing, Benxin,Yang, Qingbo,Zhu, Yingxi,&Zhao, Jiang.(2009).Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces.JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS,47(24),2541-2546.
MLA	Jing, Benxin,et al."Mobility of Single DNA Chain Under Electric Field During Its Transient Contact with Solid Surfaces".JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS 47.24(2009):2541-2546.