A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections

doi:10.1021/la901444c

CORC > 化学研究所 > 中国科学院化学研究所

	A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections
	Feng, Xuli; Xu, Qingling; Liu, Libing; Wang, Shu
刊名	LANGMUIR
	2009-12-15
卷号	25 期号:24 页码:13737-13741
ISSN号	0743-7463
DOI	10.1021/la901444c
英文摘要	A new fluorescent microgel containing the CP moiety (PFP-NIPAm) was prepared with the size within 100 nm. Covalent linking of the Conjugated polyelectrolyte moiety into the microgel prevents leakage of the fluorophore while keeping its fluorescence property. The new fluorescent microgel call be used as all optical platform to detect DNA and enzyme. More importantly, because the electrostatic attraction dominates the interactions between cationic PFP-NIPAm microgel and negatively charged target, washing With high ionic strength aqueous solution call block their interactions and displace the target from the PFP-NIPAm microgel. Thus, PFP-NIPAm can be readily reusable for detection. Another unique feature is that the PFP-NIPAm microgel extends the detection media from homogeneous solution to solid phase, which shows great potential for biodetection in the real world using conjugated polymers. Furthermore, the detection call be carried out under UV light, and no expensive detection instrumentation is needed. In principle, such all assay system Could be expanded to a high-throughput assay.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000272394400007
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66163]
专题	中国科学院化学研究所
通讯作者	Wang, Shu
作者单位	Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Feng, Xuli,Xu, Qingling,Liu, Libing,et al. A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections[J]. LANGMUIR,2009,25(24):13737-13741.
APA	Feng, Xuli,Xu, Qingling,Liu, Libing,&Wang, Shu.(2009).A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections.LANGMUIR,25(24),13737-13741.
MLA	Feng, Xuli,et al."A New Light-Harvesting Conjugated Polyelectrolyte Microgel for DNA and Enzyme Detections".LANGMUIR 25.24(2009):13737-13741.