Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column

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	Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column
	Liu Hai-Yan 1; Yang Geng-Liang 1,2; Bai Li-Gai 1; Feng Xiao-Juan 1; Yang Guan-Qun 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2009-03-01
卷号	37 期号:3 页码:325-329
关键词	Monolithic Column Separation Immunoglobulin g Immunoglobulin y
ISSN号	0253-3820
英文摘要	Using vinyl ester resin as monomer, ethylene gylcol dimethacrylate (EDMA) as the crosslinker, dodecanol as porogenic solvents and 2, 2' - azobisisobutyronitrile (AIBN) as initiators, a poly (vinyl ester resin-co-ethylene dimethacrylate) monolithic column was prepared by "in-situ" process. The monolith was further derivatized by 3-glycidoxypropyltrimethoxysilane and then hydrolyzed in 0.25 mol/L sulfuric acid to prepare diol column. The effects of pH and buffer concentration on the retention of IgG were investigated and the separation of IgG from human plasma was realized. the separation of IgG from chicken egg yolk in different buffer concentration and the effect of spacer arm on the separation were also investigated. The results showed that the monolith with spacer arm had a higher separation capacity. Moreover, the adsorption capacity of the column was examined and the maximum adsorption amount was 35 mu g/g.
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000265886400003
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/66095]
专题	中国科学院化学研究所
通讯作者	Yang Geng-Liang
作者单位	1.Hebei Univ, Coll Pharm, Hebei Prov Key Lab Pharmaceut Qual Control, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Liu Hai-Yan,Yang Geng-Liang,Bai Li-Gai,et al. Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(3):325-329.
APA	Liu Hai-Yan,Yang Geng-Liang,Bai Li-Gai,Feng Xiao-Juan,&Yang Guan-Qun.(2009).Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(3),325-329.
MLA	Liu Hai-Yan,et al."Separation of Immunoglobulin G and Immunoglobulin Y Using Poly(vinyl ester resin-co-ethylene dimethacrylate) Monolithic Column".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.3(2009):325-329.