Gating of single synthetic nanopores by proton-driven DNA molecular motors

doi:10.1021/ja800266p

CORC > 化学研究所 > 中国科学院化学研究所

	Gating of single synthetic nanopores by proton-driven DNA molecular motors
	Xia, Fan 3; Guo, Wei 1; Mao, Youdong 3; Hou, Xu 3; Xue, Jianming 1; Xia, Hongwei 3; Wang, Lin 1; Song, Yanling 3; Ji, Hang 2; Qi Ouyang 2
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2008-07-02
卷号	130 期号:26 页码:8345-8350
ISSN号	0002-7863
DOI	10.1021/ja800266p
英文摘要	Switchable ion channels that are made of membrane proteins play different roles in cellular circuits. Since gating nanopore channels made of proteins can only work in the environment of lipid membrane, they are not fully compatible to the application requirement as a component of those nanodevice systems in which lipid membranes are hard to establish. Here we report a synthetic nanopore-DNA system where single solid-state conical nanopores can be reversibly gated by switching DNA motors immobilized inside the nanopores. High- (on-state) and low- (off-state) conductance states were found within this nanopore-DNA system corresponding to the single-stranded and i-motif structures of the attached DNA motors. The highest gating efficiency indicated as current ratio of on-state versus off-state was found when the length of the attached DNA molecule matched the tip diameter of the nanopore well. This novel nanopore-DNA system, which was gated by collective folding of structured DNA molecules responding to the external stimulus, provided an artificial counterpart of switchable protein-made nanopore channels. The concept of this DNA motor-driven nanopore switch can be used to build novel, biologically inspired nanopore machines with more precisely controlled functions in the near future by replacing the DNA molecules with other functional biomolecules, such as polypeptides or protein enzymes.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000257152800050
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/63139]
专题	中国科学院化学研究所
通讯作者	Wang, Yugang
作者单位	1.Peking Univ, State Key Lab Nucl Phys & Technol, Beijing 100871, Peoples R China 2.Peking Univ, Ctr Microfluid & Nanotechnol, Beijing 100871, Peoples R China 3.Chinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xia, Fan,Guo, Wei,Mao, Youdong,et al. Gating of single synthetic nanopores by proton-driven DNA molecular motors[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2008,130(26):8345-8350.
APA	Xia, Fan.,Guo, Wei.,Mao, Youdong.,Hou, Xu.,Xue, Jianming.,...&Jiang, Lei.(2008).Gating of single synthetic nanopores by proton-driven DNA molecular motors.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,130(26),8345-8350.
MLA	Xia, Fan,et al."Gating of single synthetic nanopores by proton-driven DNA molecular motors".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130.26(2008):8345-8350.