Three-stage refolding/unfolding of the dual-color beta-subunit in R-phycocyanin from Polysiphonia urceolata

doi:10.1016/j.bbrc.2006.11.085

CORC > 化学研究所 > 中国科学院化学研究所

	Three-stage refolding/unfolding of the dual-color beta-subunit in R-phycocyanin from Polysiphonia urceolata
	Ma, Yuhui; Xie, Jie; Zhang, Chunxi; Zhao, Jingquan
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2007-01-19
卷号	352 期号:3 页码:787-793
关键词	Dual-color Beta-subunit Folding/unfolding Fret Conformation Configuration Protein-framework Movement
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2006.11.085
英文摘要	The conformational changes during refolding and unfolding of the dual-color beta-subunit in R-phycocyanin (R-PC) were monitored by the spectra, fluorescence anisotropy, and FRET. It was observed that both of the refolding and unfolding of the P-subunit would undergo a three-stage conformational change, but in a reverse order. During the refolding process, at the first stage, the configuration of the tetrapyrrole chromophores transformed from the cyclohelical to the extended one, suggested by the blue-shifted spectra. At the second stage, recovery of the hydrogen-bond and hydrophobic interaction network fixed the chromophore in a more rigid configuration, suggested by a linear increase in the total fluorescence yield. At the third stage, the increase of the FRET efficiency suggested a protein-framework movement that made the two chromophores closer or/and into a more parallel orientation. The fluorescence anisotropy further confirmed the three-stage model. (c) 2006 Elsevier Inc. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000243147000034
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/61637]
专题	中国科学院化学研究所
通讯作者	Zhao, Jingquan
作者单位	Chinese Acad Sci, BNLMS, Key Lab Photochem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Ma, Yuhui,Xie, Jie,Zhang, Chunxi,et al. Three-stage refolding/unfolding of the dual-color beta-subunit in R-phycocyanin from Polysiphonia urceolata[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,352(3):787-793.
APA	Ma, Yuhui,Xie, Jie,Zhang, Chunxi,&Zhao, Jingquan.(2007).Three-stage refolding/unfolding of the dual-color beta-subunit in R-phycocyanin from Polysiphonia urceolata.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,352(3),787-793.
MLA	Ma, Yuhui,et al."Three-stage refolding/unfolding of the dual-color beta-subunit in R-phycocyanin from Polysiphonia urceolata".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 352.3(2007):787-793.