Detection of oncoprotein platelet-derived growth factor using a fluorescent signaling complex of an aptamer and TOTO

doi:10.1007/s00216-005-0276-2

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	Detection of oncoprotein platelet-derived growth factor using a fluorescent signaling complex of an aptamer and TOTO
	Zhou, CS ; Jiang, YX ; Hou, S ; Ma, BC ; Fang, XH ; Li, ML
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2006-03-01
卷号	384 期号:5 页码:1175-1180
关键词	Aptamer Pdgf-bb Oncoprotein Toto Fluorescence
ISSN号	1618-2642
DOI	10.1007/s00216-005-0276-2
英文摘要	There have recently been advances in the application of aptamers, a new class of nucleic acids that bind specifically with target proteins, as protein recognition probes for biomedical study. The development of a signaling aptamer with the capability of simple and rapid real-time detection of disease-related proteins has attracted increasing interest. We have recently reported a new protein-detection strategy using a signaling aptamer based on a DNA molecular light-switching complex, [Ru(phen)(2)(dppz)](2+). In this work we have used the commercially available DNA-intercalating dye, TOTO, to replace [Ru(phen)(2)(dppz)](2+) for detection of oncoprotein platelet-derived growth factor BB (PDGF-BB), a potential cancer marker. Taking advantage of the high affinity of the aptamer to PDGF-BB and the sensitive fluorescence change of the aptamer-TOTO signaling complex on protein binding, PDGF-BB was detected in physiological buffer with high selectivity and sensitivity. The detection limit was 0.1 nmol L-1, which was better than that of other reported aptamer-based methods for PDGF-BB, including that using [Ru(phen)(2)(dppz)](2+). The method is very simple with no need for covalent labeling of the aptamer or probe synthesis. It facilitates wide application of the signaling mechanism to the analysis and study of cancer markers and other proteins.
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000235752700021
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/58105]
专题	中国科学院化学研究所
通讯作者	Li, ML
作者单位	1.Sichuan Univ, Coll Chem, Chengdu 610064, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Zhou, CS,Jiang, YX,Hou, S,et al. Detection of oncoprotein platelet-derived growth factor using a fluorescent signaling complex of an aptamer and TOTO[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,384(5):1175-1180.
APA	Zhou, CS,Jiang, YX,Hou, S,Ma, BC,Fang, XH,&Li, ML.(2006).Detection of oncoprotein platelet-derived growth factor using a fluorescent signaling complex of an aptamer and TOTO.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,384(5),1175-1180.
MLA	Zhou, CS,et al."Detection of oncoprotein platelet-derived growth factor using a fluorescent signaling complex of an aptamer and TOTO".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 384.5(2006):1175-1180.