G-quadruplex DNA aptamers for zeatin recognizing

doi:10.1016/j.bios.2012.08.004

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	G-quadruplex DNA aptamers for zeatin recognizing
	Qi, Cui 1,2; Bing, Tao 1; Mei, Hongcheng 1,2; Yang, Xiaojuan 1,2; Liu, Xiangjun 1; Shangguan, Dihua 1
刊名	BIOSENSORS & BIOELECTRONICS
	2013-03-15
卷号	41 页码:157-162
关键词	Aptamer Zeatin Aptasensor Selex Graphene Oxide
ISSN号	0956-5663
DOI	10.1016/j.bios.2012.08.004
英文摘要	Zeatins, a major type of cytokinin, are ubiquitous in higher plants, and involve in regulating a wide range of developmental processes. The development of highly specific ligands to zeatins would be very useful in plant biological research. Here we describe a group of oligonucleotide ligands (aptamers) generated against trans-zeatin. The optimized aptamers possess strong affinity to trans-zeatin and trans-zeatin riboside (Kd=3-5 mu M), and relatively weak affinity (Kd=27-30 mu M) to cis-zeatin and dihydrozeatin. These aptamers adopt a hairpin-G-quadruplex structure for binding to zeatin. A fluorescence turn-on aptasensor based on graphene oxide (GO) was developed for the recognition of zeatins. The specificity assay of this aptasensor shows good response to zeatins, and no response to the adenine derivatives (analog of zeatins) abundantly existing in biological samples. These results show the great potential of these aptamers in chemical analysis and biological investigation of zeatins. (C) 2012 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000314191300020
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/54521]
专题	中国科学院化学研究所
通讯作者	Shangguan, Dihua
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Qi, Cui,Bing, Tao,Mei, Hongcheng,et al. G-quadruplex DNA aptamers for zeatin recognizing[J]. BIOSENSORS & BIOELECTRONICS,2013,41:157-162.
APA	Qi, Cui,Bing, Tao,Mei, Hongcheng,Yang, Xiaojuan,Liu, Xiangjun,&Shangguan, Dihua.(2013).G-quadruplex DNA aptamers for zeatin recognizing.BIOSENSORS & BIOELECTRONICS,41,157-162.
MLA	Qi, Cui,et al."G-quadruplex DNA aptamers for zeatin recognizing".BIOSENSORS & BIOELECTRONICS 41(2013):157-162.