Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP

doi:10.1016/j.polymer.2012.11.029

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	Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP
	Zhao, Min 1; Yang, Lingye 1; Zhang, Ruichen 1; Dong, Jie 1; Dong, Haifeng 2; Wen, Yongqiang 2; Zhan, Xiaowei 3; Wang, Ge 1; Lu, YunFeng 1; Wang, Guojie 1
刊名	POLYMER
	2013-01-08
卷号	54 期号:1 页码:297-302
关键词	Hybridization Fluorescence Polyelectrolytes
ISSN号	0032-3861
DOI	10.1016/j.polymer.2012.11.029
英文摘要	A novel pyrene-labeled polyelectrolyte (Py-PDMAEMA(+)) has been prepared by atom transfer radical polymerization (ATRP) for detection of DNA hybridization. The electrostatic and hydrophobic interactions between Py-PDMAEMA(+) and ssDNA (hairpin or linear DNA) kept them forming a complex probe which could be used as a turn-off fluorescent sensor. The fluorescence intensity of the probe decreased upon adding the complementary strand, since the pyrene moiety could be intercalated into the duplex and thus the fluorescence quenching by nucleotide bases was strengthened. The proposed intercalation mode was confirmed by the circular dichroism spectra and the fluorescence quenching study with iodide. This pyrene-labeled polyelectrolyte combined with ssDNA may establish a novel fluorescence sensing system for DNA hybridization. (C) 2012 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000322356000035
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53991]
专题	中国科学院化学研究所
通讯作者	Wang, Guojie
作者单位	1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Univ Sci & Technol Beijing, Res Ctr Bioengn & Sensing Technol, Beijing 100083, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Min,Yang, Lingye,Zhang, Ruichen,et al. Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP[J]. POLYMER,2013,54(1):297-302.
APA	Zhao, Min.,Yang, Lingye.,Zhang, Ruichen.,Dong, Jie.,Dong, Haifeng.,...&Wang, Guojie.(2013).Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP.POLYMER,54(1),297-302.
MLA	Zhao, Min,et al."Detection of DNA hybridization by a pyrene-labeled polyelectrolyte prepared by ATRP".POLYMER 54.1(2013):297-302.