Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins

doi:10.1021/bc500195w

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	Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins
	Lo, Gang 1; Liu, Yu 1; Yu, Xuerong 1; Li, Xiaoyu 1,2
刊名	BIOCONJUGATE CHEMISTRY
	2014-06-01
卷号	25 期号:6 页码:1172-1180
ISSN号	1043-1802
DOI	10.1021/bc500195w
英文摘要	Characterization of small molecule (SM)-protein interaction is of high importance in biomedical research such as target identification and proteomic profiling. Photo-cross-linking is a powerful and straightforward strategy to covalently capture SM's binding proteins. The DNA-based photoaffinity labeling method is able to capture SM's protein targets with high specificity but suffers low cross-linking efficiency, which limits its utility for low abundance and low affinity proteins. After screening a variety of cross-linkers, by utilizing the multivalency effect, the cross-linking efficiency was improved by nearly 7-fold without compromising probe specificity. The generality and performance of multivalent photoaffinity probes have been validated with a variety of SM-protein pairs in the complexity of cell lysates.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000337720000017
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52829]
专题	中国科学院化学研究所
通讯作者	Li, Xiaoyu
作者单位	1.Peking Univ, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci BNLMS, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, Shenzhen Grad Sch, Sch Chem Biol & Biotechnol, Key Lab Chem Genom, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Lo, Gang,Liu, Yu,Yu, Xuerong,et al. Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins[J]. BIOCONJUGATE CHEMISTRY,2014,25(6):1172-1180.
APA	Lo, Gang,Liu, Yu,Yu, Xuerong,&Li, Xiaoyu.(2014).Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins.BIOCONJUGATE CHEMISTRY,25(6),1172-1180.
MLA	Lo, Gang,et al."Multivalent Photoaffinity Probe for Labeling Small Molecule Binding Proteins".BIOCONJUGATE CHEMISTRY 25.6(2014):1172-1180.