Insight into the fabrication of polymeric particle based oxygen carriers

doi:10.1016/j.ijpharm.2014.04.004

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	Insight into the fabrication of polymeric particle based oxygen carriers
	Li, Bin 1,2; He, Shasha 1,2; Qi, Yanxin 1; Xie, Zhigang 1; Chen, Xuesi 3; Jing, Xiabin 1; Huang, Yubin 1
刊名	INTERNATIONAL JOURNAL OF PHARMACEUTICS
	2014-07-01
卷号	468 期号:1-2 页码:75-82
关键词	Hemoglobin Emulsification Polymeric Particle Burst Release Cross-linking Blood Compatibility
ISSN号	0378-5173
DOI	10.1016/j.ijpharm.2014.04.004
英文摘要	For the sake of protein stability and targeted application as blood substitutes, formulation customization of hemoglobin-loaded polymeric particles (HbP) was conducted via a double emulsion method. Screening of the emulsification parameters was firstly performed for the stability of Hb, and the structure and functions of recovered Hb could be well preserved via CD and UV-vis spectroscopy investigation. In the optimized conditions, Hb was loaded into the polymeric matrix formed of three material compositions. They were poly(e-caprolactone)(PCL), poly(ethylene glycol)-block-poly(allyl glycidyl ether) (functionalized with mercaptopropionic acid)-block-poly(e-caprolactone) (PEG-PAGE(MPA)-PCL), and the blend of the two polymers. The morphology, internal structure, in vitro leakage and hemocompatibility of the HbP products were characterized in detail, and the encapsulation mechanism was explored by the combined analysis of the encapsulation efficiency, non-specific protein adsorption and in vitro leakage studies. Results showed that the burst release effect found in homopolymers could be alleviated by use of block copolymers due to the reduced protein adsorption, and completely avoided by further cross-linking of particles through carbonyl-amino condensation reactions. The amphiphilic copolymers showed relatively high stability in blood and no interference with blood components compared with hydrophobic PCL. These results suggest that both the optimization of emulsion formation and material composition are prerequisite for stable formulations of Hb encapsulated in polymeric particles. (C) 2014 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000336486600008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52767]
专题	中国科学院化学研究所
通讯作者	Huang, Yubin
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Li, Bin,He, Shasha,Qi, Yanxin,et al. Insight into the fabrication of polymeric particle based oxygen carriers[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2014,468(1-2):75-82.
APA	Li, Bin.,He, Shasha.,Qi, Yanxin.,Xie, Zhigang.,Chen, Xuesi.,...&Huang, Yubin.(2014).Insight into the fabrication of polymeric particle based oxygen carriers.INTERNATIONAL JOURNAL OF PHARMACEUTICS,468(1-2),75-82.
MLA	Li, Bin,et al."Insight into the fabrication of polymeric particle based oxygen carriers".INTERNATIONAL JOURNAL OF PHARMACEUTICS 468.1-2(2014):75-82.