Explicit Differentiation of G-Quadruplex/Ligand Interactions: Triplet Excited States as Sensitive Reporters

doi:10.1021/jz501040a

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	Explicit Differentiation of G-Quadruplex/Ligand Interactions: Triplet Excited States as Sensitive Reporters
	Song, Di; Yang, Wen; Qin, Tingxiao; Wu, Lidan; Liu, Kunhui; Su, Hongmei
刊名	JOURNAL OF PHYSICAL CHEMISTRY LETTERS
	2014-07-03
卷号	5 期号:13 页码:2259-2266
ISSN号	1948-7185
DOI	10.1021/jz501040a
英文摘要	We report a new transient spectral method utilizing triplet excited state as sensitive reporters to monitor and differentiate the multiplex G-quadruplex/ligand interactions in a single assay, which is a difficult task and usually requires a combination of several techniques. From a systematic study on the interactions of porphyrin (TMPyP4) with each telomeric G-quadruplex: AG(3)(T(2)AG(3))(3), G(2)T(2)G(2)TGTG(2)T(2)G(2), (G(4)T(4)G(4))(2), and (TG(4)T)(4), it is convincingly shown that the ligand triplet decay lifetimes are sensitive to the local bound microenviromnent within G-quadruplexes, from which the coexisting binding modes of end-stacking, intercalation, and sandwich are distinguished and their respective contribution are determined. The complete scenario of mixed interaction modes is thus revealed, shedding light on the past controversial issues. Additional control experiments demonstrate the sensitivity of this triplet reporter method, which can even capture the binding behavior change as the G-quadruplex structures are adjusted by Na+ or K+.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000338693200016
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/51885]
专题	中国科学院化学研究所
通讯作者	Su, Hongmei
作者单位	Chinese Acad Sci, Inst Chem, State Key Lab Mol React Dynam, BNLMS, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Song, Di,Yang, Wen,Qin, Tingxiao,et al. Explicit Differentiation of G-Quadruplex/Ligand Interactions: Triplet Excited States as Sensitive Reporters[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2014,5(13):2259-2266.
APA	Song, Di,Yang, Wen,Qin, Tingxiao,Wu, Lidan,Liu, Kunhui,&Su, Hongmei.(2014).Explicit Differentiation of G-Quadruplex/Ligand Interactions: Triplet Excited States as Sensitive Reporters.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,5(13),2259-2266.
MLA	Song, Di,et al."Explicit Differentiation of G-Quadruplex/Ligand Interactions: Triplet Excited States as Sensitive Reporters".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 5.13(2014):2259-2266.