A protein engineered to bind uranyl selectively and with femtomolar affinity

doi:10.1038/NCHEM.1856

CORC > 化学研究所 > 中国科学院化学研究所

	A protein engineered to bind uranyl selectively and with femtomolar affinity
	Zhou, Lu 1,2; Bosscher, Mike 1,2; Zhang, Changsheng 3,4,5; Oezcubukcu, Salih 1,2; Zhang, Liang 1,2; Zhang, Wen 1,2; Li, Charles J.1,2; Liu, Jianzhao 1,2; Jensen, Mark P.6; Lai, Luhua 3,4,5
刊名	NATURE CHEMISTRY
	2014-03-01
卷号	6 期号:3 页码:236-241
ISSN号	1755-4330
DOI	10.1038/NCHEM.1856
英文摘要	Uranyl (UO22+), the predominant aerobic form of uranium, is present in the ocean at a concentration of similar to 3.2 parts per 10(9) (13.7 nM); however, the successful enrichment of uranyl from this vast resource has been limited by the high concentrations of metal ions of similar size and charge, which makes it difficult to design a binding motif that is selective for uranyl. Here we report the design and rational development of a uranyl-binding protein using a computational screening process in the initial search for potential uranyl-binding sites. The engineered protein is thermally stable and offers very high affinity and selectivity for uranyl with a K-d of 7.4 femtomolar (fM) and >10,000-fold selectivity over other metal ions. We also demonstrated that the uranyl-binding protein can repeatedly sequester 30-60% of the uranyl in synthetic sea water. The chemical strategy employed here may be applied to engineer other selective metal-binding proteins for biotechnology and remediation applications.
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000331951800014
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/51593]
专题	中国科学院化学研究所
通讯作者	Lai, Luhua
作者单位	1.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 2.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA 3.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, BNLMS, Beijing 100871, Peoples R China 4.Peking Univ, Ctr Quantitat Biol, Beijing 100871, Peoples R China 5.Peking Univ, Ctr Life Sci, Beijing 100871, Peoples R China 6.Argonne Natl Lab, Chem Sci & Engn Div, Argonne, IL 60439 USA
推荐引用方式 GB/T 7714	Zhou, Lu,Bosscher, Mike,Zhang, Changsheng,et al. A protein engineered to bind uranyl selectively and with femtomolar affinity[J]. NATURE CHEMISTRY,2014,6(3):236-241.
APA	Zhou, Lu.,Bosscher, Mike.,Zhang, Changsheng.,Oezcubukcu, Salih.,Zhang, Liang.,...&He, Chuan.(2014).A protein engineered to bind uranyl selectively and with femtomolar affinity.NATURE CHEMISTRY,6(3),236-241.
MLA	Zhou, Lu,et al."A protein engineered to bind uranyl selectively and with femtomolar affinity".NATURE CHEMISTRY 6.3(2014):236-241.