Analysis of the Steps in Single-Molecule Photobleaching Traces by Using the Hidden Markov Model and Maximum-Likelihood Clustering

doi:10.1002/asia.201402147

CORC > 化学研究所 > 中国科学院化学研究所

	Analysis of the Steps in Single-Molecule Photobleaching Traces by Using the Hidden Markov Model and Maximum-Likelihood Clustering
	Yuan, Jinghe; He, Kangmin; Cheng, Ming; Yu, Jianqiang; Fang, Xiaohong
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2014-08-01
卷号	9 期号:8 页码:2303-2308
关键词	Fluorescence Hidden Markov Model Photobleaching Maximum-likelihood Clustering Single-molecule Studies
ISSN号	1861-4728
DOI	10.1002/asia.201402147
英文摘要	The step analysis of single-molecule photobleaching data offers a new approach for studying protein stoichiometry under physiological conditions. As such, it is important to develop suitable algorithms that can accurately extract the step events from the noisy single-molecule data. Herein, we report a HMM method that combines maximum-likelihood clustering for initializing the emission-probability distribution of the HMMs with an extended silhouette clustering criterion for estimating the state number of single molecules. In this way, the limitations of standard HMM in terms of processing typical single-molecule data with a short sequence are overcome. By using this method, the number and time points of the step events are automatically determined, without the introduction of any subjectivity. Simulation experiments on the experimental photobleaching data indicate that our method is very effective and robust in the analysis of single-molecule fluorescence photobleaching curves if the signal/noise ratio is larger than 2:1. This method was employed for processing photobleaching data that were obtained from single-molecule fluorescence imaging of transforming growth factor typeII receptors on a cell surface. This method is also expected to be applicable to the analysis of other stepwise events.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000342675900044
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/51225]
专题	中国科学院化学研究所
通讯作者	Yuan, Jinghe
作者单位	Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Jinghe,He, Kangmin,Cheng, Ming,et al. Analysis of the Steps in Single-Molecule Photobleaching Traces by Using the Hidden Markov Model and Maximum-Likelihood Clustering[J]. CHEMISTRY-AN ASIAN JOURNAL,2014,9(8):2303-2308.
APA	Yuan, Jinghe,He, Kangmin,Cheng, Ming,Yu, Jianqiang,&Fang, Xiaohong.(2014).Analysis of the Steps in Single-Molecule Photobleaching Traces by Using the Hidden Markov Model and Maximum-Likelihood Clustering.CHEMISTRY-AN ASIAN JOURNAL,9(8),2303-2308.
MLA	Yuan, Jinghe,et al."Analysis of the Steps in Single-Molecule Photobleaching Traces by Using the Hidden Markov Model and Maximum-Likelihood Clustering".CHEMISTRY-AN ASIAN JOURNAL 9.8(2014):2303-2308.