Cancer Cell Uptake of Polymer Hydrogel Nanotubes

doi:10.1166/jbn.2014.1923

CORC > 化学研究所 > 中国科学院化学研究所

	Cancer Cell Uptake of Polymer Hydrogel Nanotubes
	Wang, Jing 1; Liu, Lixin 2; Zhu, Wen 1; Chen, Yongming 1,4; Wang, Chun 3
刊名	JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
	2014-11-01
卷号	10 期号:11 页码:3329-3336
关键词	Cancer Cells Cellular Uptake Polymer Nanotubes
ISSN号	1550-7033
DOI	10.1166/jbn.2014.1923
英文摘要	To gain deeper understanding of how the shape and size of particles influence their interaction with cells, the uptake process of polymer hydrogel nanotubes (PNTs) by cancer cells was characterized. The PNTs were composed of crosslinked poly(ethylene glycol) segments with a diameter of 300 nm and a polydispersed length in the range of 2 to 20 mu m. The uptake of PNTs into human cervical carcinoma epithelial (HeLa) cells cultured in vitro was monitored by confocal laser fluorescence microscopy and details of PNT entry into cells were revealed by a series of confocal Z-section images and time-lapsed video microscopy. It was found that PNTs initiating contact with the cell membrane through the tips of the tubes were readily taken up by the cells, whereas the uptake of tubes approaching the cell surface in parallel manner was very difficult. The length of the tubes did not appear to affect cellular uptake. In fact, it was found surprisingly that PNTs as long as > 10 mu m were internalized by these non-phagocytic cells. The rate of PNT uptake varied significantly and was probably influenced by the angle of attachment. Better understanding of the interaction between PNTs and cells may provide important insight in the use of PNTs as potential anticancer drug delivery vehicles.
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000339430900012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50387]
专题	中国科学院化学研究所
通讯作者	Liu, Lixin
作者单位	1.Chinese Acad Sci, Inst Chem, Lab Polymer Phys & Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Univ Minnesota, Dept Biomed Engn, Minneapolis, MN 55455 USA 4.Sun Yat Sen Univ, Sch Chem & Chem Engn, Dept Polymer & Mat Sci, Key Lab Minist Educ Polymer Composite & Funct Mat, Guangzhou 510275, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jing,Liu, Lixin,Zhu, Wen,et al. Cancer Cell Uptake of Polymer Hydrogel Nanotubes[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2014,10(11):3329-3336.
APA	Wang, Jing,Liu, Lixin,Zhu, Wen,Chen, Yongming,&Wang, Chun.(2014).Cancer Cell Uptake of Polymer Hydrogel Nanotubes.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,10(11),3329-3336.
MLA	Wang, Jing,et al."Cancer Cell Uptake of Polymer Hydrogel Nanotubes".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 10.11(2014):3329-3336.