Novel Oligo(ethylene glycol)-Based Molecularly Imprinted Magnetic Nanoparticles for Thermally Modulated Capture and Release of Lysozyme

doi:10.1021/am505427j

CORC > 化学研究所 > 中国科学院化学研究所

	Novel Oligo(ethylene glycol)-Based Molecularly Imprinted Magnetic Nanoparticles for Thermally Modulated Capture and Release of Lysozyme
	Li, Nan 1,2,3; Qi, Li 1,2; Shen, Ying 1,2,3; Qiao, Juan 1,2; Chen, Yi 1,2
刊名	ACS APPLIED MATERIALS & INTERFACES
	2014-10-08
卷号	6 期号:19 页码:17289-17295
关键词	Oligo(Ethylene Glycol)-based Monomers Thermoresponsive Moleculary Imprinted Polymer Magnetic Nanoparticles Surface Imprinting Lysozyme
ISSN号	1944-8244
DOI	10.1021/am505427j
英文摘要	In this study, oligo(ethylene glycol) (OEG)-based thermoresponsive molecularly imprinted polymers (MIPs) for lysozyme on the surface of magnetic nanoparticles were synthesized. Thermoresponsive monomer 2-(2-methoxyethoxy)ethyl methacrylate, chelate monomer N-(4-vinyl)-benzyl iminodiacetic acid, and acidic monomer methacrylic acid were selected as the ingredients for preparing the MIP layer. The thermoresponsive behavior of the novel imprinted magnetic nanoparticles was evaluated by dynamic light scattering and swelling ratios measurements. Interestingly, in analysis of lysozyme, the capture/release process could be modulated by changing the temperature, avoiding tedious washing steps. Meanwhile, high adsorption capacity (204.1 mg/g) and good selectivity for capturing lysozyme were achieved. Additionally, surface imprinting with magnetic nanoparticles as substrate allowed for short adsorption time (2 h) and rapid magnetic separation. Furthermore, the proposed imprinted magnetic nanoparticles were used to selectively extract lysozyme in human urine with recoveries ranging from 89.2% to 97.3%. The results indicated that the OEG-based monomers are promising for responsive MIP preparation, and the proposed imprinted material is efficient for thermally modulated capture and release of target protein.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000343018200097
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50311]
专题	中国科学院化学研究所
通讯作者	Qi, Li
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Nan,Qi, Li,Shen, Ying,et al. Novel Oligo(ethylene glycol)-Based Molecularly Imprinted Magnetic Nanoparticles for Thermally Modulated Capture and Release of Lysozyme[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(19):17289-17295.
APA	Li, Nan,Qi, Li,Shen, Ying,Qiao, Juan,&Chen, Yi.(2014).Novel Oligo(ethylene glycol)-Based Molecularly Imprinted Magnetic Nanoparticles for Thermally Modulated Capture and Release of Lysozyme.ACS APPLIED MATERIALS & INTERFACES,6(19),17289-17295.
MLA	Li, Nan,et al."Novel Oligo(ethylene glycol)-Based Molecularly Imprinted Magnetic Nanoparticles for Thermally Modulated Capture and Release of Lysozyme".ACS APPLIED MATERIALS & INTERFACES 6.19(2014):17289-17295.