Construction of glycoprotein multilayers using the layer-by-layer assembly technique

doi:10.1039/c2jm33070a

CORC > 化学研究所 > 中国科学院化学研究所

	Construction of glycoprotein multilayers using the layer-by-layer assembly technique
	Wang, Bo 1,2; Liu, Zhiyong 3; Xu, Yanli 3; Li, Yuhua 1; An, Tiezhu 1; Su, Zhaohui 2; Peng, Bo 2; Lin, Yuan 2; Wang, Qian 4
刊名	JOURNAL OF MATERIALS CHEMISTRY
	2012
卷号	22 期号:34 页码:17954-17960
ISSN号	0959-9428
DOI	10.1039/c2jm33070a
英文摘要	A novel glycoprotein film was assembled from pig gastric mucin and poly(acrylamide-co-3-acrylamidophenylboronic acid) using a layer-by-layer technique, driven by the formation of boronate ester bonds between the boronic acid units and the polyols. The assembly was monitored by quartz crystal microbalance and UV-vis spectroscopy. The film thickness is increased with increasing the ionic strength and the pH of assembly solutions. Furthermore, the dynamic response of the assembled film to the presence of glucose was monitored in real time using quartz crystal microbalance with dissipation. Glucose competes with pig gastric mucin to bind with the boronic acid units in the multilayer film, resulting in its disassembly, and the disassembly rate is a function of the glucose concentration. The film is also sensitive to glucose at physiological conditions, albeit the response is weaker and slower than that at higher pH. This glucose-sensitive glycoprotein multilayer film can therefore be applied in glucose sensing and self-regulated insulin release systems.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000307305700079
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/49851]
专题	中国科学院化学研究所
通讯作者	An, Tiezhu
作者单位	1.NE Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 3.Chinese Acad Sci, NE Inst Geog & Agroecol, Changchun 130012, Peoples R China 4.Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
推荐引用方式 GB/T 7714	Wang, Bo,Liu, Zhiyong,Xu, Yanli,et al. Construction of glycoprotein multilayers using the layer-by-layer assembly technique[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(34):17954-17960.
APA	Wang, Bo.,Liu, Zhiyong.,Xu, Yanli.,Li, Yuhua.,An, Tiezhu.,...&Wang, Qian.(2012).Construction of glycoprotein multilayers using the layer-by-layer assembly technique.JOURNAL OF MATERIALS CHEMISTRY,22(34),17954-17960.
MLA	Wang, Bo,et al."Construction of glycoprotein multilayers using the layer-by-layer assembly technique".JOURNAL OF MATERIALS CHEMISTRY 22.34(2012):17954-17960.