Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance

doi:10.1016/j.cclet.2012.10.017

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	Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance
	Wang, Hai Yan 1; Li, Jian Jun 2; Cao, Xiao Na 3; Xu, Ji Ying 4; Liu, Mei Rong 4; Chen, Yi 4
刊名	CHINESE CHEMICAL LETTERS
	2012-12-01
卷号	23 期号:12 页码:1393-1395
关键词	Hemodialyzed Patients Cd4(+) T-lymphocytes Antibody-antigen Interactions Spr
ISSN号	1001-8417
DOI	10.1016/j.cclet.2012.10.017
英文摘要	A surface plasmon resonance (SPR) method was presented to discriminate hemodialyzed T-lymphocytes from the normal based on antibody cell recognition. By dynamic reaction with fixed anti-human CD4 antibody, SPR could offer significant signals to distinguish hemodialyzed patients from the healthy Controls within 200 s after the cell injection in respect of either rising speed or maximum binding capacity (p < 0.01). The ratio method is also used to exclude the non-specific adsorption. The percentage of hemodialyzed patients' CD4(+) T cells against the healthy control is 69 +/- 18%. The most attractive of the present method is its ability to detect the intact and label-free lymphocytes, and further to detect the subpopulations, or proteins secreted by the desired lymphocytes subset. (C) 2012 Yi Chen. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000313999900021
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/49577]
专题	中国科学院化学研究所
通讯作者	Chen, Yi
作者单位	1.Shanxi Datong Univ, Sch Chem & Chem Engn, Datong 037009, Peoples R China 2.Dongzhimen Hosp Affiliated, East Sect, Beijing 101100, Peoples R China 3.Being Sport Univ, Community Hlth Serv, Beijing 100190, Peoples R China 4.ICCAS, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hai Yan,Li, Jian Jun,Cao, Xiao Na,et al. Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance[J]. CHINESE CHEMICAL LETTERS,2012,23(12):1393-1395.
APA	Wang, Hai Yan,Li, Jian Jun,Cao, Xiao Na,Xu, Ji Ying,Liu, Mei Rong,&Chen, Yi.(2012).Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance.CHINESE CHEMICAL LETTERS,23(12),1393-1395.
MLA	Wang, Hai Yan,et al."Detection of CD4(+) T-lymphocytes from hemodialyzed patients by surface plasmon resonance".CHINESE CHEMICAL LETTERS 23.12(2012):1393-1395.