A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications

doi:10.1073/pnas.1113770109

CORC > 化学研究所 > 中国科学院化学研究所

	A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications
	Chang, Hao 1,2; Zhang, Mingshu 1,5; Ji, Wei 1; Chen, Juanjuan 3; Zhang, Yongdeng 4; Liu, Bei 4; Lu, Jingze 1; Zhang, Junlong 3; Xu, Pingyong 1; Xu, Tao 1,4
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2012-03-20
卷号	109 期号:12 页码:4455-4460
关键词	(f)Palm/storm Meos2 Mgeos Superresolution Microscopy
ISSN号	0027-8424
DOI	10.1073/pnas.1113770109
英文摘要	Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection.
语种	英语
出版者	NATL ACAD SCIENCES
WOS记录号	WOS:000301712600028
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47709]
专题	中国科学院化学研究所
通讯作者	Xu, Pingyong
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Peking Univ, Coll Chem & Mol Engn, State Key Lab Rare Earth Mat Chem & Applicat, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 4.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
推荐引用方式 GB/T 7714	Chang, Hao,Zhang, Mingshu,Ji, Wei,et al. A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(12):4455-4460.
APA	Chang, Hao.,Zhang, Mingshu.,Ji, Wei.,Chen, Juanjuan.,Zhang, Yongdeng.,...&Xu, Tao.(2012).A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(12),4455-4460.
MLA	Chang, Hao,et al."A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.12(2012):4455-4460.