In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation

doi:10.1007/s11426-012-4567-0

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	In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation
	Xin Ying 1; Zhang ZiPin 2; Yu Ping 2; Ma FuRong 1; Mao LanQun 2
刊名	SCIENCE CHINA-CHEMISTRY
	2013-02-01
卷号	56 期号:2 页码:256-261
关键词	On-line Electrochemical Detection In Vivo Microdialysis Magnesium Vertigo
ISSN号	1674-7291
DOI	10.1007/s11426-012-4567-0
英文摘要	Vertigo is one of the most common clinical symptoms. However, the chemical processes involved in the pathological mechanism of vertigo remain to be fully understood. In this study, we investigate the dynamic changes in the magnesium (Mg2+) concentration in medial vestibular nucleus (MVN) of guinea pigs following vertigo induced by vestibular ice water stimulation with an electrochemical detection method consisting of in vivo microdialysis and on-line selective electrochemical detection. Electrochemical detection of Mg2+ was accomplished based on the current enhancement of Mg2+ towards the electrocatalytic oxidation of NADH at the electrodes modified with the polymerized film of toluidine blue O (TBO). Selectivity for the on-line electrochemical detection against Ca2+ was achieved by using ethyleneglcol-bis(2-aminoethylether) tetraacetic acid (EGTA) as the selective masking agent for Ca2+. The basal level of the extracellular Mg2+ in the MVN of guinea pigs was determined to be 759.7 +/- 176.2 mu M(n = 16). Upon ice water irrigation of the left external ear canal, the concentration of Mg2+ in the MVN decreases significantly, reaches 72 +/- 6% (n = 8) of the basal level, and maintains for at least 1000 s. Control experiments reveal that neither warm water irrigation of the external ear canal nor ice water irrigation of the auricle induces the decrease in the concentration of Mg2+ in the MVN. These results demonstrate that the extracellular Mg2+ in the MVN decreases significantly following vertigo induced by vestibular ice water stimulation. This demonstration suggests that Mg2+ might play an important role in the pathological mechanism of vertigo.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000314271900012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/46959]
专题	中国科学院化学研究所
通讯作者	Ma FuRong
作者单位	1.Peking Univ, Hosp 3, Dept Otorhinolaryngol, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xin Ying,Zhang ZiPin,Yu Ping,et al. In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation[J]. SCIENCE CHINA-CHEMISTRY,2013,56(2):256-261.
APA	Xin Ying,Zhang ZiPin,Yu Ping,Ma FuRong,&Mao LanQun.(2013).In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation.SCIENCE CHINA-CHEMISTRY,56(2),256-261.
MLA	Xin Ying,et al."In vivo electrochemical recording of continuous change of magnesium in medial vestibular nucleus following vertigo induced by ice water vestibular stimulation".SCIENCE CHINA-CHEMISTRY 56.2(2013):256-261.