Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels

doi:10.1016/j.carbpol.2018.01.035

CORC > 化学研究所 > 中国科学院化学研究所

	Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels
	Wei, Xing 1,2; Xiong, Hejian 1,2; Zhou, Dongfang 1; Jing, Xiabin 1; Huang, Yubin 1
刊名	CARBOHYDRATE POLYMERS
	2018-04-15
卷号	186 页码:45-53
关键词	Neutral Protein Nanogels Sodium Alginate Ion-assisted Method Bioactivity
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2018.01.035
英文摘要	Protein crosslinked nanogels which combine the merits of nanogels and the specific biological activity from protein have emerged as fascinating protein delivery systems. However, the fragility and low density of reactive group in proteins, especially low electric density of neutral proteins seriously limited the fabrication of protein crosslinked nanogels without affecting their bioactivities. Here, we demonstrated a facile ion-assisted method to fabricate neutral protein crosslinked nanogels. Oxidized sodium alginate (OSA) with aldehyde groups and low viscosity was successfully synthesized, which could reversibly form nanogels via addition and removal of divalent cation. Subsequently, hemoglobin and myoglobin were used as representative neutral proteins to fabricate stable protein crosslinked OSA nanogels under the assist of divalent cation followed by in situ Schiff base formation between OSA and proteins. The mild fabrication condition guaranteed the structural integrity and bioactivity of proteins in the obtained protein crosslinked nanogels. This ion-assisted method was expected to bring a new opportunity for fabricating versatile functional biohybrid nanogels systems.
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000426360800006
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/45683]
专题	中国科学院化学研究所
通讯作者	Zhou, Dongfang; Huang, Yubin
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Jilin, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wei, Xing,Xiong, Hejian,Zhou, Dongfang,et al. Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels[J]. CARBOHYDRATE POLYMERS,2018,186:45-53.
APA	Wei, Xing,Xiong, Hejian,Zhou, Dongfang,Jing, Xiabin,&Huang, Yubin.(2018).Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels.CARBOHYDRATE POLYMERS,186,45-53.
MLA	Wei, Xing,et al."Ion-assisted fabrication of neutral protein crosslinked sodium alginate nanogels".CARBOHYDRATE POLYMERS 186(2018):45-53.