Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush

doi:10.1007/s10118-018-2031-0

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	Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush
	Zhao, Jie 1; Mo, Ru 3; Tian, Li-Mei 1; Song, Ling-Jie 2; Luan, Shi-Fang 2; Yin, Jing-Hua 2; Ren, Lu-Quan 1
刊名	CHINESE JOURNAL OF POLYMER SCIENCE
	2018-04-01
卷号	36 期号:4 页码:472-478
关键词	Biomimetic Dopamine Atom Transfer Radical Polymerization (Atrp) Boronic Acid (Ba) Immunoassay Biomimetic
ISSN号	0256-7679
DOI	10.1007/s10118-018-2031-0
英文摘要	High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness, it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work, copolymer of zwitterionic sulfobetaine methacrylate (SBMA) and glycidyl methacrylate (GMA) brushes were immobilized onto inert polypropylene (PP) via surface-initiated atom transfer radical polymerization (ATRP) based on biomimetic dopamine pretreatment. Subsequently, boronic acid (BA) groups were covalently bonded via active GMA units, followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush, the bioactivity of antibody is well preserved, which endows the surface with significantly enhanced antigen-binding capacity. Moreover, the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption, significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000426311800004
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/45649]
专题	中国科学院化学研究所
通讯作者	Zhao, Jie
作者单位	1.Jilin Univ, Minist Educ, Key Lab Bion Engn, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 3.Jilin Prov Peoples Hosp, Changchun 130021, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jie,Mo, Ru,Tian, Li-Mei,et al. Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush[J]. CHINESE JOURNAL OF POLYMER SCIENCE,2018,36(4):472-478.
APA	Zhao, Jie.,Mo, Ru.,Tian, Li-Mei.,Song, Ling-Jie.,Luan, Shi-Fang.,...&Ren, Lu-Quan.(2018).Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush.CHINESE JOURNAL OF POLYMER SCIENCE,36(4),472-478.
MLA	Zhao, Jie,et al."Oriented Antibody Immobilization and Immunoassay Based on Boronic Acid-containing Polymer Brush".CHINESE JOURNAL OF POLYMER SCIENCE 36.4(2018):472-478.