A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles

	A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles
	Qu, Fei ; Huang, Wei ; You, Jinmao
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2018-02-01
英文摘要	In this work, we report a fluorescence strategy for detecting dopamine (DA) and sensing tyrosinase (TYR) activity on the basis of the dual-emission carbon dots (DECDs), which contain two emitters: the blue emitters (BE, maximum emission at 385 nm) and yellow emitters (YE, maximum emission at 530 nm). Gold nanoparticles (AuNPs) can effectively quench the two emissions of DECDs. The addition of DA aggregates AuNPs effectively, leading to the fluorescence recovery of dual emitters gradually. This strategy exhibits a high selectivity toward DA and shows good linear ranges, such as 0.5-3 mu M for BE and 0.1-3 mu M for YE. Additionally, the proposed method is successfully applied to the determination of DA in real samples with satisfactory recoveries. Subsequently, this DECDs-AuNPs platform is further taken advantage to assess TYR activity by the aid of TYR's capability for oxidation of DA into dopaquinone, which will not induce the agglomeration of AuNPs, so the fluorescence quenching of DECDs is associated with TYR activity. Finally, the mechanism of the reaction is discussed in detail, and the results suggest that both amine and phenolic hydroxyl groups of DA bring the aggregation of AuNPs. (C) 2017 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/13331]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Qu, Fei,Huang, Wei,You, Jinmao. A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2018.
APA	Qu, Fei,Huang, Wei,&You, Jinmao.(2018).A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles.COLLOIDS AND SURFACES B-BIOINTERFACES.
MLA	Qu, Fei,et al."A fluorescent sensor for detecting dopamine and tyrosinase activity by dual-emission carbon dots and gold nanoparticles".COLLOIDS AND SURFACES B-BIOINTERFACES (2018).