Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect

	Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect
	Lu, Shuaimin ; Li, Guoliang ; Lv, Zhengxian ; Qiu, Nannan ; Kong, Weiheng ; Gong, Peiwei ; Chen, Guang ; Xia, Lian ; Guo, Xiaoxi ; You, Jinmao ; Wu, Yongning
刊名	BIOSENSORS & BIOELECTRONICS
	2016-11-15
英文摘要	Early detection and diagnosis have great practical significances for the effective prevention and treatment of cancer. In this study, we developed a novel, facile and ultra-sensitive fluorescence assay for the determination of tumor invasive biomarker beta-glucuronidase (GLU) based on the inner-filter effect (IFE). The nitrogen-doped carbon quantum dots (N-CQDs) with green photoluminescence were employed as the fluorophore in IFE, and 4-nitrophenyl-beta-D-glucuronide (PNPG) was used to act as GLU substrate, and GLU catalytic product (p-nitrophenol (PNP)) was capable of acting as the robust absorber in IFE to turn off the fluorescence of N-CQDs due to the complementary overlap between the absorption of PNP and the excitation of N-CQDs. Thus, signal of GLU activity could be recorded by the fluorescence intensity of N-CQDs. Unlike other fluorescence sensing mechanism such as fluorescence resonance energy transfer (FRET) or photoinduced electron transfer (PET), IFE has no requirement for electron or energy transfer process or any chemical modification of fluorophore, which makes our assay more flexible and simple. The proposed method exhibited a good linear relationship from 1 U L-1 to 60 U L-1 (R-2=0.9967) with a low detection limit of 0.3 U L-1. This method was also successfully applied to the analysis of serum samples and the inhibitor screening from natural product. The developed sensor platform was proven to be reliable, facile, sensitive, and selective, making it promising as a candidate for GLU activity detection in clinic tumor diagnose and anti-tumor drug screening. (C) 2016 Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/6444]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Lu, Shuaimin,Li, Guoliang,Lv, Zhengxian,et al. Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect[J]. BIOSENSORS & BIOELECTRONICS,2016.
APA	Lu, Shuaimin.,Li, Guoliang.,Lv, Zhengxian.,Qiu, Nannan.,Kong, Weiheng.,...&Wu, Yongning.(2016).Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect.BIOSENSORS & BIOELECTRONICS.
MLA	Lu, Shuaimin,et al."Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect".BIOSENSORS & BIOELECTRONICS (2016).