Multiple comparisons of three different sources of biomaterials in the application of tumor tissue engineering in vitro and in vivo

doi:10.1016/j.ijbiomac.2019.02.136

	Multiple comparisons of three different sources of biomaterials in the application of tumor tissue engineering in vitro and in vivo
	Li, Wenfang 1; Hu, Xueyan 1; Wang, Shuping 2,3; Xing, Yan 4; Wang, Hai 1; Nie, Yi 5,6; Liu, Tianqing 1; Song, Kedong 1
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2019-06-01
卷号	130 页码:166-176
关键词	3D tumor model Decellularized lung matrix Chitosan/gelatin Poly-L-lactic acid Cell response Drug screening
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2019.02.136
英文摘要	Potential anti-cancer drugs are frequently of low efficacy in clinics due to the lack of predictive models or the insufficient employment of existing preclinical test systems. Three-dimensional (3D) in vitro engineered tumor models can better predict the efficacy of novel drugs by reproducing the in vivo tumor microenvironment. In this study, three sources of scaffolds (decellularized lung scaffold, chitosan/gelatin scaffold, and poly-L-lactic acid scaffold) incorporated with breast cancer cells (MCF-7, 4T1) were bioengineered as a platform to study in vitro solid tumor development The good biocompatibility of three scaffolds favored cell growth and proliferation. Cells in 3D scaffolds were less sensitive to chemotherapy and exhibited characteristics of higher malignancy compared to their 2D counterparts. The expression of breast cancer biomarkers in MCF-7 cells markedly up-regulated in 3D scaffolds in comparison with those in 2D cultures. Cells grown in 3D scaffolds were found to be more tumorigenic and angiogenic in BABL/c mice xenografts than cells grown from monolayers. The results demonstrate that 3D engineered tumor model can better mimic in vivo tumor and can serve as a more appropriate platform for the study and screening of novel cancer therapeutics. (C) 2019 Published by Elsevier B.V.
资助项目	National Natural Science Foundation of China[31670978/31370991/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning Province[20180510028]
WOS关键词	SCAFFOLDS ; CANCER ; FABRICATION ; CELLS ; DRUG
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000466253000018
资助机构	National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning Province
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/28177]
专题	中国科学院过程工程研究所
通讯作者	Song, Kedong
作者单位	1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 1, Regenerat Med Ctr, Dalian 116024, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 1, Stem Cell Clin Res Ctr, Dalian 116024, Peoples R China 4.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China 5.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 6.Chinese Acad Sci, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Li, Wenfang,Hu, Xueyan,Wang, Shuping,et al. Multiple comparisons of three different sources of biomaterials in the application of tumor tissue engineering in vitro and in vivo[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,130:166-176.
APA	Li, Wenfang.,Hu, Xueyan.,Wang, Shuping.,Xing, Yan.,Wang, Hai.,...&Song, Kedong.(2019).Multiple comparisons of three different sources of biomaterials in the application of tumor tissue engineering in vitro and in vivo.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,130,166-176.
MLA	Li, Wenfang,et al."Multiple comparisons of three different sources of biomaterials in the application of tumor tissue engineering in vitro and in vivo".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 130(2019):166-176.