Functional Single-Cell Approach to Probing Nitrogen-Fixing Bacteria in Soil Communities by Resonance Raman Spectroscopy with N-15(2) Labeling

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	Functional Single-Cell Approach to Probing Nitrogen-Fixing Bacteria in Soil Communities by Resonance Raman Spectroscopy with N-15(2) Labeling
	Zhu, Yong-Guan; Cui, Li; Yang, Kai; Li, Hong-Zhe; Zhang, Han; Su, Jian-Qiang; Paraskevaidi, Maria; Martin, Francis L.; Ren, Bin
刊名	ANALYTICAL CHEMISTRY
	2018-04-17
卷号	90 期号:8 页码:5082-5089
ISSN号	0003-2700
文献子类	Article
英文摘要	Nitrogen (N) fixation is the conversion of inert nitrogen gas (N-2) to bioavailable N essential for all forms of life. N-2-fixing microorganisms (diazotrophs), which play a key role in global N cycling, remain largely obscure because a large majority are uncultured. Direct probing of active diazotrophs in the environment is still a major challenge. Herein, a novel culture-independent single-cell approach combining resonance Raman (RR) spectroscopy with N-15(2) stable isotope probing (SIP) was developed to discern N-2-fixing bacteria in a complex soil community. Strong RR signals of cytochrome c (Cyt c, frequently present in diverse N-2-fixing bacteria), along with a marked N-15(2)-induced Cyt c band shift, generated a highly distinguishable biomarker for N-2 fixation. N-15(2)-induced shift was consistent well with N-15 abundance in cell determined by isotope ratio mass spectroscopy. By applying this biomarker and Raman imaging, N-2-fixing bacteria in both artificial and complex soil communities were discerned and imaged at the single-cell level. The linear band shift of Cyt c versus N-15(2) percentage allowed quantification of N-2 fixation extent of diverse soil bacteria. This single-cell approach will advance the exploration of hitherto uncultured diazotrophs in diverse ecosystems.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/41221]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Zhu, Yong-Guan,Cui, Li,Yang, Kai,et al. Functional Single-Cell Approach to Probing Nitrogen-Fixing Bacteria in Soil Communities by Resonance Raman Spectroscopy with N-15(2) Labeling[J]. ANALYTICAL CHEMISTRY,2018,90(8):5082-5089.
APA	Zhu, Yong-Guan.,Cui, Li.,Yang, Kai.,Li, Hong-Zhe.,Zhang, Han.,...&Ren, Bin.(2018).Functional Single-Cell Approach to Probing Nitrogen-Fixing Bacteria in Soil Communities by Resonance Raman Spectroscopy with N-15(2) Labeling.ANALYTICAL CHEMISTRY,90(8),5082-5089.
MLA	Zhu, Yong-Guan,et al."Functional Single-Cell Approach to Probing Nitrogen-Fixing Bacteria in Soil Communities by Resonance Raman Spectroscopy with N-15(2) Labeling".ANALYTICAL CHEMISTRY 90.8(2018):5082-5089.